Assessment Residency Post-Assignment 1

1. Assessment Residency Post-Assignment 1 • The unit I chose to work on is antibiotic function/resistance. It is later on in the semester (mid-oct) • It is my first time teaching this microbiology class. (100-150 students) • I have included some ideas for clicker questions/exam questions/activities, but will adjust as I learn more about my students • I have already implemented a couple of successful things in earlier units • Clicker questions for participation credit • Study guides with learning objectives • Online homework with pre/post unit components • Printed in class activities for them to write on

2. Fundamental Statement: Bacteria have unique cell structures that can be targets for antibiotics

3. Fundamental Statement: Genetic mutations and horizontal gene transfer, together with selective pressure, lead to the rise of antibiotic resistance

4. Students will understand what makes a good antimicrobial drug and how drug resistance spreads in a population Overarching Goals of the antimicrobial drug unit ……Understand the features of an effective antimicrobial drug ……explain how not completing a full treatment of antibiotics can lead to an increase in resistance in a bacterial population Students will be able to *…to analyze bacterial growth assays to determine antibacterial drug resistance Intermediate steps *…predict what type of cells a drug will target *…..list the structures unique to prokaryotic cells *..explain how antibiotic resistance spreads in a population of cells Building Blocks Information flow Genetic Mutation Horizontal Gene transfer Prokaryotic cell structure Metabolism Eukaryotic cell structure

5. ….. Summative Exam at Unit End (F) In class activities Oct 12-16, 2015 …. Build on lower level outcomes …. Smaller group activity (TPS) (E) Lecture with clickers Address problems from homework (D) Mon Oct 12 Wed Oct 14 Fri Oct 16 Sat/Sun Tues Thurs Sat/Sun Adaptive Homework: Problem Solving Due Sunday 11pm (C ) Read (A) Online Homework: Fact finding Due Sunday 11pm (B) *150-200 Bio Majors So-Sr Out of class activities

6. A. Read (Study Guide) • I generate a study guide for each unit. This is an example from the first unit • I will generate the study guide for this unit a week or so before (Oct 5-7)

7. B/C: Homework • These assignments include two components. A simple set of questions on the reading for the next unit, and a problem based set of questions for the previous unit • Example reading q (Blooms ½): What does the minimum inhibitory concentration (MIC) of a chemical tell you? A)It tells you the smallest concentration of the chemical that is needed to inhibit the growth of any microorganism. B)It tells you the smallest concentration of the chemical that is needed to kill a specific microorganism. C)It tells you the smallest concentration of an organism that is needed for chemical control. D)It tells you the smallest concentration of the chemical that is needed to inhibit the growth of a specific microorganism. • Example Follow up q (problem based): Attached is the case study. It is followed by a guided series of questions. From Pearson’s MasteringMicrobiology

8. D) In class clickers on reading/lecture 1:Oct 12 • This question is related to the learning objective “Students will be able to predict, based on the function of a drug, what type of organism it will affect” The antibiotic cycloheximide binds to the 60S subunit of the ribosome as shown in Figure 20.2. The effect is to A) Prevent mRNA-ribosome binding in eukaryotes. B) Prevent peptide bond formation in prokaryotes. C) Prevent polypeptide elongation in eukaryotes. D) Prevent transcription in prokaryotes. E) Prevent ribosome formation in bacteria.

9. E) In class activity (Lecture 2): Oct 14 • I am currently working on adjusting the following case study to meet my in class needs. I am shortening sections 2 and 3 to fit within a 50 minute class. I will address: • Mechanisms of resistance • Evolution of resistance • http://sciencecases.lib.buffalo.edu/cs/collection/detail.asp?case_id=594&id=594

10. F) Summative Exam (Oct 16) • I do not have any TAs for my 150 students, so my exams are multiple choice • I’ve attached some lower order questions to address my learning objectives • I am working on generating higher order questions to include on the exam. I usually include a mix of comprehension and apply/analyze on each exam. • Objective 1: An antimicrobial that inhibits cell wall synthesis will result in which of the following? • A) Cells become more susceptible to osmotic pressure. • B) Cells cannot attach to their hosts. • C) Ribosomes lose their function. • D) The sterols in the cell wall become nonfunctional. • E) The replication of cells, including cancer cells, slows down.

11. F) Summative Exam (Oct 16) • Objective 2: Which drug is least likely to impact the cells of the human host? • A) A drug that targets the plasma membrane • B) A drug that targets peptidoglycan synthesis • C) A drug that targets a component of the 70S ribosome • D) A drug that targets a component the 80S ribosome

12. F) Summative Exam (Oct 16) • Objective 3: Antibiotic resistance can spread from one bacterium to another via direct bacterial cell contact through the process of • A) Transduction • B) Transformation • C) Recombination • D) Conjugation

13. F) Summative Exam (Oct 16) • Objective 4:The overuse and misuse of antimicrobial drugs leads to antibiotic-resistant bacteria because • A) the few surviving bacteria that are affected by the antibiotics develop immunity to the antibiotics, which they pass on to their progeny. • B) the antibiotics persist in the environment • C) the antibiotics cause new mutations to occur in the surviving bacteria, which results in resistance to antibiotics. • D) the antibiotics kill susceptible bacteria, but the few that are naturally resistant live and reproduce, and their progeny survive and grow