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Chapter 9: Cellular Respiration

Chapter 9: Cellular Respiration. Objectives The student is responsible for: The definitions of all bold faced words in the chapter Knowing the entire chapter. The student is not responsible for: Memorizing or drawing the structures of glycolysis or Kreb’s cycle.

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Chapter 9: Cellular Respiration

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  1. Chapter 9: Cellular Respiration • Objectives • The student is responsible for: • The definitions of all bold faced words in the chapter • Knowing the entire chapter. • The student is not responsible for: • Memorizing or drawing the structures of glycolysis or Kreb’s cycle

  2. Principles of Energy Harvest Fermentation: decomposition of glucose without the use of oxygen Cellular Respiration: oxygen is a reactant when glucose is broken down

  3. There is an integral relationship between photosynthesis and respiration. Figure 9.1 Energy flow and chemical recycling in ecosystems The production of ATP is an exergonic process

  4. Figure 9.x1 ATP Adenosine Triphosphate ATP -> ADP + Pi ADP  AMP + Pi

  5. Why do we care so much about ATP? Figure 9.2 A review of how ATP drives cellular work

  6. Figure 9.3 Methane combustion as an energy-yielding redox reaction Oxidizing Agent: that substance that is being reduced. O is “going” from O (no charge) to O2-. Reducing Agent: that substance that is being oxidized. C is gaining oxygen.

  7. Various foods can be oxidized to produce ATP. Figure 9.19 The catabolism of various food molecules

  8. The molecule that is used to move hydrogen ions throughout the oxidation of food is NAD+. Therefore NAD+ is an oxidizing agent. Figure 9.4 NAD+ as an electron shuttle NAD+ + H+ NADH

  9. Figure 9.5 An introduction to electron transport chains

  10. Figure 9.6 An overview of cellular respiration (Layer 1) But if we could get this pyruvate into the mitochondria we could make a whole lot more ATP!!

  11. Figure 9.6 An overview of cellular respiration (Layer 2)

  12. Figure 9.6 An overview of cellular respiration (Layer 3)

  13. Figure 9.7 Substrate-level phosphorylation What is this substrate-level phosphorylation? This is when a phosphate group is moved from an organic compound to ADP. What is oxidative phosphorylation? When electrons and H+ are used to make ATP.

  14. Figure 9.8 The energy input and output of glycolysis Couldn’t these NADH’s that are made be used to make ATP?

  15. Figure 9.9 A closer look at glycolysis: energy investment phase (Layer 1)

  16. Figure 9.9 A closer look at glycolysis: energy investment phase (Layer 2)

  17. Figure 9.9 A closer look at glycolysis: energy payoff phase (Layer 3)

  18. Figure 9.9 A closer look at glycolysis: energy payoff phase (Layer 4)

  19. Figure 9.10 Conversion of pyruvate to acetyl CoA, the junction between glycolysis and the Krebs cycle Could the NADH produced here be used to make ATP?

  20. Figure 9.11 A closer look at the Krebs cycle (Layer 1) Keep track of the number of carbons!!

  21. Figure 9.11 A closer look at the Krebs cycle (Layer 2) More NADHs!!!

  22. Figure 9.11 A closer look at the Krebs cycle (Layer 3)

  23. Figure 9.11 A closer look at the Krebs cycle (Layer 4)

  24. Figure 9.12 A summary of the Krebs cycle

  25. NADH and FADH2 deliver electrons to different locations in the ETC. Figure 9.13 Free-energy change during electron transport The role of oxygen is to serve as a hydrogen ion acceptor to form water.

  26. ATP Synthase Chemiosmosis: the coupling of the movement of H+ through a protein complex (ATP Synthase) making ATP. Figure 9.14 ATP synthase, a molecular mill

  27. Figure 9.15 Chemiosmosis couples the electron transport chain to ATP synthesis A Proton-Motive Force is produced

  28. Figure 9.16 Review: how each molecule of glucose yields many ATP molecules during cellular respiration

  29. Figure 9.17a Fermentation

  30. Figure 9.17b Fermentation

  31. Figure 9.18 Pyruvate as a key juncture in catabolism

  32. Power Bars? Luna Bars? Promax? Goo? Figure 9.19 The catabolism of various food molecules

  33. Versatility of Catabolism • Use of Proteins • Proteins  amino acids and then the amino acids must have their amino groups removed before being used as an energy source. So all the energy bars that have amino acids in them are at least one step closer to being used for energy than a protein. • Fats must go through beta oxidation which takes a fat and breaks off 2 carbon fragments from the fatty acids and these 2 carbon fragments enter at acetyl-CoA.

  34. Figure 9.20 The control of cellular respiration  • The Control of Cellular Respiration • PFK: allosteric enzyme • Receptor sites for ATP, AMP and citrate • ATP: inhibitor • AMP: stimulator • Citrate: inhibitor

  35. The Evolutionary Significance of Glycolysis Earliest organisms were in an anaerobic environment (3.5 billion yrs ago) Glycolysis was probably used as an energy making process Oxygen accumulated about 2.7 billion years ago Glycolysis is the most widespread pathway amongst organisms suggesting it evolved early on. Glycolysis requires only the cytoplasm and membrane-bound organelles were not present until eukaryotic cells appeared (2 billion years after prokaryotes)

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