1 / 17

Data Blitz

Data Blitz. Eric Spivey 30 March 2011. Biopolymer Solution. Background: Using pulsed laser-generated multiphoton excitation to fabricate micron scale hydrogel matrices from biopolymer solutions. Biopolymer Hydrogel Matrix. Focused, pulsed laser. Microscope Objective.

zonta
Download Presentation

Data Blitz

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. Data Blitz Eric Spivey 30 March 2011

  2. Biopolymer Solution Background: Using pulsed laser-generated multiphoton excitation to fabricate micron scale hydrogel matrices from biopolymer solutions. Biopolymer Hydrogel Matrix Focused, pulsed laser Microscope Objective Motivation: Use this method to fabricate cell scaffolds for neural tissue engineering applications. Goal:Fabricate hydrogel microstructures with tunable functional and mechanical properties.

  3. Multiphoton fabricated PEG-DA hydrogels • Why PEG? • FDA approved biomaterial, non-immunogenic • Widely used in tissue engineering studies as a hydrogel • Widely used in drug delivery for tunable release properties • Issues • Not naturally degradable • Tricky solubility in aqueous solutions at low MW • Fairly strong negative charge

  4. poly(ethyleneglycol) diacrylate (PEGDA, average mw ~700 Da) PEG structures =13 25 µm 25 µm

  5. Fabricating PEG structure using a Mask

  6. Using ESEM

  7. Functionalization of PEG with BP-biotin 2 mW 3mW 4mW 5 mW

  8. Indentation Modeling Force = F Radius R Indentation = d • Can calculate Young’s Modulus with F, R and d, • Assume: • Poisson’s ratio is 0.5 and • Substrate measured is infinitely thick • Corrections exist for non-infinite substrates

  9. Setup for Validating Finite Thickness Model Δh ~8 µm Glass PVA Force Map (3x)

  10. Validation of finite-thickness Hertz Model

  11. BSA+GMHA Modulus(20% BSA w/v)

  12. PEG + GMHA Modulus(60% PEG v/v)

  13. PEG + GMHA Modulus(30% PEG v/v)

  14. NG 108 cells in PEG structures

  15. Questions?

  16. Talk on Thursday  "Neuroscience on a Chip" Albert Folch Professor, Department of Bioengineering University of Washington Thursday, March 31, 2011 at 3:30 PM NHB 1.720

  17. Stage Light Effect

More Related