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Purdue iGEM 2012

Purdue iGEM 2012. Meeting Tuesday 6/19/12. Recap of the Past Week’s Work. Transformations Minipreps Not ordering all of OmpA – Silicatein Experimental Design Methods Eric McLamore Reading Papers. Mini Prep. Plan for the Next Week. 3A Assembly

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Purdue iGEM 2012

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  1. Purdue iGEM 2012 Meeting Tuesday 6/19/12

  2. Recap of the Past Week’s Work • Transformations • Minipreps • Not ordering all of OmpA – Silicatein • Experimental Design Methods • Eric McLamore • Reading Papers

  3. Mini Prep

  4. Plan for the Next Week • 3A Assembly • Start designing experiments based on methods from today • Split up work for WIKI

  5. Meeting Notes: • Eric McLamore – what exactly is your project? More details so we could use it as a control • Quorum system • Does he have a control system • What is his area of expertese? • Steve is back in his office – wants to talk to him • Human Practices are talking to him Thurs • Call and find out if we can present our project thus far • E-mail stats professors • How do we want to synthesize OmpA? • Just making OmpA with Gibson the rest is using 3A • Sean made the primers in the dropbox

  6. Plan for the Next Week • Tuning the system with different strains of promoter and RBS • In biobrick workshop – ask Soo • Just use the ones we have • Mini prep Tuesday • Transforming Wed and another Mini prep • Transformation #2 Thurs • Mini prep #2 Fri • Weekend Transformation #3 • Mon or Tues done with Transformation • Max and Mrudula meet with Janie about varialbes for model in JMP • Send to Amanda, Rubeena, Rachel to start designing experiments to run

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