1 / 4

Electron Microscopy - Osler Institute Pathology Board Review

Electron Microscopy - Osler Institute Pathology Board Review. Objectives Detect and recognize cytoplasmic organelles and matrix Interpret the ultrastructural features of commonly encountered tumors, non neoplastic entities and infectious agents Guide to tissue submission

Sophia
Download Presentation

Electron Microscopy - Osler Institute Pathology Board Review

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. Electron Microscopy - Osler Institute Pathology Board Review • Objectives • Detect and recognize cytoplasmic organelles and matrix • Interpret the ultrastructural features of commonly encountered tumors, non neoplastic entities and infectious agents • Guide to tissue submission • Undifferentiated malignant tumors Small cell malignancies • Neuroendocrine tumors CNS tumors • Anomalous IHC results Select IHC panel • Eosinophilic granuloma Renal biopsies • Heart biopsies Nerve biopsies • Bullous diseases of skin Storage diseases • Rapid diagnosis of viral infection Ciliary dysmorphology • Extracellular matrix

  2. Electron Microscopy - Osler Institute Pathology Board Review • Processing tissue for electron microscopy • 1 mm cubed tissue pieces • Dehydrate, infiltrate, osmicate, embed in plastic • Toluidine blue thick sections • Thin sections – 100 nm on copper grids (silver sections) • Interpretation of electron micrographs • Determine approximate magnification • Identify organelles • Integrate ultrastructural features with clinical history, light microscopy, IHC and molecular diagnostics • Limitations of electron microscopy • Sample size • Multidirectional differentiation • No diagnostic organelles

  3. Electron Microscopy - Osler Institute Pathology Board Review • Primer of Organelles • Nucleus • Abundant heterochromatin – Ewings sarcoma, B cell lymphoma • Cerebriform nuclear contours • Nuclear pseudoinclusions – nuclear pockets • Intranuclear tubular aggregates • Nucleolus • Meandering reticular nucleoli – seminoma, dysgerminoma • Marginated Nucleolus • Increased nucleoli Slide 3

  4. Electron Microscopy - Osler Institute Pathology Board Review • Primer of Organelles • Cytoplasm • Annulate lamellae Cylindrical confronting cisternae • Cytoskeleton Endoplasmic reticulum • Golgi Intercellular junctions • Lamellar cytoplasmic inclusions Lipid • Langerhan’s cell granules Lysosomes • Melanosomes Microvilli • Mitochondria Ribosomes and polyribosomes • Secretory granules Tubular reticular structures • Weibel Palade bodies • Extracellular Matrix • Amyloid • Collagen

More Related