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Work package 1 (WP1) Topic: Cyanobacterial Dormant Forms in an Aquatic environment

Work package 1 (WP1) Topic: Cyanobacterial Dormant Forms in an Aquatic environment Objective: Establishment of dormant stage / Induction of akinetes in cyanobacteria. Akinete formation – triggers and processes. # D4 and # D8

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Work package 1 (WP1) Topic: Cyanobacterial Dormant Forms in an Aquatic environment

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  1. Work package 1 (WP1) Topic: Cyanobacterial Dormant Forms in an Aquatic environment Objective: Establishment of dormant stage/ Induction of akinetes in cyanobacteria

  2. Akinete formation – triggers and processes

  3. # D4 and # D8 • Basic studies to reveal conditions and mechanisms involved in induction of akinetes formation, dormancy and desiccation: Nutrient • Depletion (P), Light, Temp, O2 (hypoxia) • Specialized envelopes • Storage of metabolites [carbohydrates, cyanophycin (N)] – enzymes involved, e.g cyanophycine synthase • Tolerance to stress: heat shock proteins/chaperones-function in maintaining structural integrity of biomolecules. • Tryptophan, aspartate, aspargin, phenyl-alanine and serine triggered akinete formation. • Mature akinetes • Respiration and Photosynthetic apparatus (is there pigments production?) • O2 evolution/consumption • Reduced energy production? • To collect adequate material for further genomic, proteomic and metabolomic studies.

  4. Genetic regulation of Akinete

  5. # D5, # D6, # D7 • Development of molecular tools for cyanobacteria • Genes that are expressed • Subtractive c-DNA libraries from A. ovalisporum • Akinetes ascompared to a culture producing akinetes and to a culture without • akinete production • Expression studies • Identification of proteins found specifically in akinetes • Metabolomic profiling for carbohydrates associated with dormancy

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