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Vanderbilt Research Shared Resources and Core Facilities

Vanderbilt Research Shared Resources and Core Facilities. Susan Meyn Director – Research Resources VUMC Office of Research. Agenda. Role of the Office of Research VUMC Strategic Plan and Core Facilities VUMC Core Facilities – Overview

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Vanderbilt Research Shared Resources and Core Facilities

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  1. Vanderbilt Research Shared Resources and Core Facilities Susan Meyn Director – Research Resources VUMC Office of Research

  2. Agenda Role of the Office of Research VUMC Strategic Plan and Core Facilities VUMC Core Facilities – Overview How VUMC Core Facilities can support your Research Efforts VUMC Core Facilities – Highlights Resources

  3. Role of the Office of REsearch

  4. Office of Research:General resources and support for research • Point of contact for the research community both on campus and outside the institution • Funds and coordinates development of specialized infrastructure (equipment, facilities) to support multiple research efforts • Provides grant-writing resources including grant-ready text • Coordinates limited submission opportunities and institutional support for selected grant programs contact email: s.meyn@vanderbilt.edu website: http://vanderbilt.edu/oor/

  5. Office of Research

  6. Office of Research:Core Facilities and Shared Resources • Supports scientific oversight and development of new shared resources, continued development of existing cores • Works with research centers and programs to keep core facilities on the cutting edge by providing new equipment, recruiting technical staff. • Coordinates with VICTR funding program to provide research support. • Oversees and administers the Scholarship program in conjunction with NIH-funded centers to provide assistance with core service fees. contact email: s.meyn@vanderbilt.edu website: http://vanderbilt.edu/oor/

  7. VUMC Strategic Plan and Core Facilities

  8. Vanderbilt’s Research Enterprise Strategic Plan and Core Facilities • Personalized Health & Healthcare • Therapeutic Discovery & Translation • Public Health & Healthcare • Foundations of Basic Science Leverages network of shared core facilities in Centers developed through expansion of space, faculty, and $100M investment. 2005–2010 VANDERBILT UNIVERSITY MEDICAL CENTER RESEARCH ENTERPRISE STRATEGIC PLAN http://www.vanderbilt.edu/oor/about/strategic-plan12-2005

  9. Vanderbilt’s Research Enterprise Strategic Plan and Core Facilities • Personalized Health & Healthcare • Therapeutic Discovery & Translation • Public Health & Healthcare • Foundations of Basic Science Leverages network of shared core facilities in Centers developed through expansion of space, faculty, and $100m liquidation of quasi-endowment (2000-2005). 2005–2010 VANDERBILT UNIVERSITY MEDICAL CENTER RESEARCH ENTERPRISE STRATEGIC PLAN http://www.vanderbilt.edu/oor/about/strategic-plan12-2005

  10. Research cores/shared resources Discovery Individual biomarkers Genomics Proteomics Metabolomics Imaging Integrative science Disease pathways Drug targets Model systems • Personalized Medicine • Disease susceptibility • Early detection & prevention • Variable drug actions Implementation Translation

  11. VUMC Core Facilities – Overview

  12. Overview – Vanderbilt Institutional Cores Medical Research Building III (MRBIII) Mass Spectrometry Resource Center Neurochemistry Core Laboratory Biomolecular Crystallography Facility Biophysical Instrumentation Core Facility Cell Imaging Shared Resource Medical Center North (MCN) CRC–Clinical Research Center Division of Animal Care Comparative Pathology Biostatistics Collaboration Center Flow Cytometry and Immunology Cores Medical Center North (MCN) Sleep Research Core Murine Neurobehavioral Laboratory Rat Neurobehavioral Laboratory Vanderbilt University Institute of Imaging Science Relocating soon (VANTAGE): Genome Sciences Resource & BioVU DNA Resources Medical Research Building IV (MRBIV) Transgenic Mouse/ESC Shared Resource VICB Chemical Synthesis Core Cell Imaging Shared Resource Light Hall Cell Imaging Shared Resource Molecular Cell Biology Resource Cores Mouse Metabolic Phenotyping Center ZCORE - Zebrafish Aquatic Facility Robinson Research Building VICB High-Throughput Screening Facility The Vanderbilt Clinic (TVC) Human Tissue Acquisition and Pathology Investigational Drug Services Preston Research Building Cancer Biostatistics Center Epidemiology Biospecimen Core Hormone Assay and Analytical Services Core VICB Protein and Antibody Resource Not shown: Computational Genomics Genetic Studies Ascertainment Survey Research Shared Resource ACCRE - Advanced Computing Center Biomolecular NMR Facility

  13. Research core or shared resource: Is a research laboratory accessible to multiple investigators across disciplines. Provides specialized or technologically advanced laboratory services, equipment and expertise. Often based in an NIH funded center, e.g. the Cancer Center. Dedicated personnel include scientific manager/director and technical staff. Supported by chargebacks to investigator grants.

  14. Typical Core Structure

  15. How VUMC Core Facilities can support your Research Efforts

  16. VUMC Core Facilities support your Research Efforts • More than 30 institutional cores and shared resources – • Whatever your research interest, there is a core facility that can offer high end equipment access, specialized technology or advanced scientific expertise. • $1 of every $10 funded by NIH goes to cores at Vanderbilt. • Vanderbilt is nationally recognized for the quality and efficiency of its core facilities. • Vanderbilt is a leader in core technology development. • Scientists at all levels come to Vanderbilt because of our cores – make these valuable resources part of your research plan.

  17. VUMC Core Facilities support your Research Efforts – Grant Funding • Grant applications – collaborate with a core: • Plan your project and define the scope of services • Request core service fees in your budget • Do not request personnel costs – i.e. no % effort • Reviewers are very positive about using core facilities to accomplish research aims.

  18. VUMC Core Facilities support your Research Efforts – Technical Support • Experimental design, planning, troubleshooting: • Not sure how to start? • Take a specific core training session • Attend a core seminar • Stop by the core to talk with core technical staff • Not finding the technology that you need? Contact the Office of Research, or search the core listings. • Core facilities help you achieve your research goals.

  19. Support to pay core service charges VUMC Core Facilities support your Research Efforts - • Unfortunately, use of research core facilities is not free! BUT there are sources of funding that can help you pay the bill: • Join a research center - for example: • Cancer Center • Diabetes Center • Vision Research Center • Digestive Disease Research Center • Members of research centers are eligible for core scholarships – credit vouchers that can be used to pay core service charges in supported core facilities. • Apply for pilot project funding or micro-grant vouchers from VICTR. Investigators may receive up to $2000 every six months to pay for core services.

  20. VUMC Core Facilities – Highlights

  21. Imaging • Center for Small Animal Imaging – MRI, PET, SPECT, CT, Ultrasound • Center for Human Studies – MRI and full range of ancillary equipment • Cell Imaging Shared Resource – Confocal laser scanning microscopes, TEM, image processing workstations

  22. Cell Imaging Shared Resource

  23. Cell Imaging Shared Resource

  24. Proteomics Mass Spectrometry Resource Center – Mass Spec, Proteomics Tissue Imaging Center for Structural Biology – NMR, X-ray crystallography, Biophysical instrumentation Institute for Chemical Biology – High Throughput Screening and Chemical Synthesis

  25. Mass Spectrometry Resource Center in rat, 2 hr post-dose Optical image Olanzapine m/z 313256 N-desmethyl- m/z 299256 2-hydroxymethyl- m/z 329272 Khatib-Shahidi, S. et al., Anal Chem, 2006, 78, 6448 http://pubs.acs.org/doi/pdf/10.1021/ac060788p

  26. Cell-based Assays, Animal Models Flow Cytometry & Immunology cores – cell surface and organelle markers, cytokine assays Transgenic Mouse – Embryonic Stem Cell Shared Resource Mouse and Rat Neurobehavior cores

  27. Flow Cytometry Core Figure 2: Distribution of memory, homing, activation and exhaustion markers. Cells from CSF (A) and blood (B) of a HIV+ patient were directly stained with a 14-colour antibody panel. Flow cytometric plots of total CD8+ T cells (in blue) are shown with HIV-specific CD8+ tetramer+ (B7-TL10) T cells on the Y axis (in green) and memory maturation markers (CD45RO, CD62L and CD127), homing markers (CCR5, VLA-4), activation markers (CD38) or markers of T cell exhaustion (PD-1 and CD57) on the X-axis. Percentages on bottom and top of each plot represent frequencies of markers on total CD8+ T cells and HIV-specific T cells respectively. • Enhanced PD-1 expression by T cells in cerebrospinal fluid does not reflect functional exhaustion during chronic human immunodeficiency virus type 1 infection. • Sadagopal S, Lorey SL, Barnett L, Sutherland D, Basham R, Erdem H, Kalams SA, Haas DW. • J Virol. 2010 Jan;84(1):131-40. Epub.

  28. Genomics • DNA Resources – extract, bank and genotype DNA • BioVU - DNA and de-identified clinical databank • Genome Sciences Resource – microarrays, next-gen sequencing (HiSeq, MiSeq)

  29. Resources

  30. Vanderbilt University Postdoctoral Research and Shared Resources Symposium Wednesday, May 2nd, 2012 Light Hall North Lobby and Langford Lobby • Invited Presentations: 10:30 am - 1:00 pm, LH 208 • * Lunch tickets provided for first 50 attendees prior to 10:30 am * • 10:30 am Dr. David Piston • Louis B. McGavock Chair, Professor of Molecular Physiology & Biophysics, Physics, & Chemical & Biomolecular Engineering • 11:00 am Dr. Kevin Schey • Director, VVRC Proteomics Core Facility • 11:15 am Dr. Daniel Foster • Vanderbilt Center for Neuroscience Drug Discovery“Elucidation of M5-muscarinic receptor • neurophysiology in midbrain dopamine neurons using • the novel allosteric modulator VU0238429” • 11:30 am Dr. Christopher Coldren • Operations Director, Genomic Sciences Resource • 11:45 am Dr. Xiaojing Wang • Department of Biomedical Informatics • “customProDB: an R package to generate customized protein database from RNA-Seq data for proteomics search” • 12:00 pm 15 minute break • Lunch provided for first 50 attendees in LH 208 • 12:15 pm Dr. Sam Wells • Director, Cell Imaging Shared Resource • 12:30 pm Dr. Thomas Kehl-Fie • Department of Pathology, Microbiology & Immunology • “Manganese sequestration by the host is an essential component of innate immunity” • 12:45 pm 15 minute break • Poster Sessions: 1:00 pm - 3:00 pm, LH Lobby & Langford Lobby * Light refreshments will be provided in Langford Lobby* • Session A, LH Lobby Session B, Langford Lobby • 1:00 - 2:00 pm 2:00 - 3:00 pm • Animal/Clinical Models Cell Biology • Biochemistry Microbiology/Immunology • Biological Sciences Molecular Physiology & Biophysics • Cancer Biology Neuroscience • Pharmacology • Keynote Address: 3:30 pm - 4:30 pm, LH 208 • Dr. Gregory S. Kopf • Associate Vice Chancellor of Research Administration, • University of Kansas Medical Center • Former Assistant Vice President at Wyeth Pharmaceuticals • “Weathering the “Perfect Storm”: Charting the Course for a Career • in the Life Sciences ” • Awards Ceremony: 4:30 pm, LH 208 • Dr. Richard Peek, 2011 Mentor of the Year • “Perspectives & Philosophies on Mentoring” • Presentation of “Mentor of the Year” Award • Presentation of “Postdoc of the Year” Award • Poster awards: “Best in Category” • “Best Use of Shared Resources” Many thanks to our generous Vanderbilt sponsors! BRET Office of Postdoctoral Affairs & Roger Chalkley Office of Research & Shared Resources Vanderbilt Medical Alumni Association Postdoctoral T32 Training Grant Directors

  31. http://vanderbilt.edu/oor/ Core Listings

  32. http://vanderbilt.edu/oor/ Grant ready text, etc

  33. https://starbrite.vanderbilt.edu/

  34. C.O.R.E.S. Core Ordering and Reporting Enterprise System www.cfuis.mc.vanderbilt.edu Web-interface Place orders directly with core facilities Reserve time on core equipment Review and act on charges made against your grants or VICTR awards

  35. Questions?

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