Need

1. Need http://personalpages.manchester.ac.uk/staff/roy.goodacre/learning/metabprof/levy_nature2004.pdf Many bacteria are both capable of developing their own resistance to antibiotics as well as spreading this resistance to other bacteria Literature Review Secondary Metabolites Synthesis of pisatin in Pisum sativum, L, var. Little Marvel (Dwarf Pea) http://www.scielo.br/img/fbpe/bjm/v31n4/a03fig03.gif Graph D shows the minimal inhibitory concentration of plant extracts and phytochemicals against antibiotic resistant bacteria. Oils and essential extracts from plants continued to be effective against even resistant microorganisms (Nascimento et. al, 00). http://pubs.acs.org/cgi-bin/sample.cgi/bipret/2008/24/i02/pdf/bp0703329.pdf Literature Review Literature Review Graphs A, B, and C show HPLC profiles from pea hairy roots after treatment with electric current (a), 0.08 mM copper chloride (b), or no treatment (c). Sub-lethal levels of electric current act as elicitors in transgenic and non-transgenic plant tissue (Kaimoyo et. al, 08). http://phytomedics.com/news/papers/poulev_etal2003.pdf Table 2 shows the effects of elicitation on the antimicrobial activity of plant root extracts. In almost all model organisms antimicrobial activity was either sustained or improved (Poulev, et. al, 03). http://pubs.acs.org/cgi-bin/sample.cgi/bipret/2008/24/i02/pdf/bp0703329.pdf Purpose The purpose of this experiment is to view the effects of altered sub-lethal levels of electric shock on the elicitation of secondary metabolites in Pisum sativum, L, var. Little Marvel Hypothesis Null Hypothesis: With respect to elicitation of secondary metabolites, the frequency distribution of values will be similar for all groups Alternate Hypothesis: Groups treated with sub-lethal electric current will express significantly higher elicitation of secondary metabolites than the untreated