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Optogenetic regulation of Ca 2+ signaling April 15, 2013

Optogenetic regulation of Ca 2+ signaling April 15, 2013. Class objectives: What is optogenetics? Mammalian rhodopsin. Biophysics of channelrhodopsin-2. Opto- XR Optically-induced dimerization . What is optogenetics?.

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Optogenetic regulation of Ca 2+ signaling April 15, 2013

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  1. Optogenetic regulation of Ca2+ signalingApril 15, 2013 Class objectives: What is optogenetics? Mammalian rhodopsin. Biophysics of channelrhodopsin-2. Opto-XR Optically-induced dimerization

  2. What is optogenetics? “Optogenetics is the combination of optical and molecular strategies to monitor and control designated molecular and cellular activities in living tissues and cells using genetically encoded photosensitive proteins.”

  3. Mammalian rhodopsin 11-cis-retinal

  4. 11-cis-retinylidene All-trans-retinylidene

  5. Channelrhodopsin-2 is a light-activated cation channel Green alga Chlamydomonas reinhardtii Nagel G et al. PNAS 2003;100:13940-13945

  6. Action spectra of channelrhodopsins Nagel G et al. PNAS 2003;100:13940-13945

  7. ChR chimeras with different spectral properties Prigge M et al. J. Biol. Chem. 2012;287:31804-31812

  8. Dual light excitation and ion selectivity for blue and green light-absorbing ChRs. Prigge M et al. J. Biol. Chem. 2012;287:31804-31812

  9. Signalling specificity and in vivo functionality of Opto-XRs RD Airanet al. Nature (2009)

  10. OptoXR: optogenetic control of intracellular signal transduction. RD Airanet al. Nature000, 1-5 (2009) doi:10.1038/nature07926

  11. Opto-a1AR activation of TRPV4 channels

  12. Systems for optically-induced protein interactions

  13. Light-induced translocation of PKCa FKF1-GI system

  14. Rapid light-induced recruitment of a 5-ptase to the plasma membrane Idevall-Hagren O et al. PNAS 2012;109:E2316-E2323

  15. Blue light-induced recruitment of a 5-phosphatase to the plasma membrane rapidly decreases KCNQ2/3 current. Idevall-Hagren O et al. PNAS 2012;109:E2316-E2323

  16. Intermission

  17. Jia et al.(2011) Fig. 1

  18. Jia et al.(2011) Fig. 2a-b

  19. Jia et al.(2011) Fig. 2c-e

  20. Jia et al.(2011) Fig. 3a-c

  21. Jia et al.(2011) Fig. 3d-f

  22. Jia et al.(2011) Fig. 4a-b

  23. Jia et al.(2011) Fig. 3c-g

  24. Jia et al.(2011) Supplemental Fig. 1a-b

  25. Jia et al.(2011) Supplemental Fig. 1c-d

  26. Jia et al.(2011) Supplemental Movies Supplemental movies

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