1 / 8

Gut Microbes Lactose Intolerance

Gut Microbes Lactose Intolerance. Introduction. Our Goals. From last week, we had three possible ways to tackle lactose intolerance: Convert H2 or methane into less harmful gases Make a super sugar metabolizing machine Engineer a strain to NOT convert glucose + galactose into H2 and methane.

brandee-canute
Download Presentation

Gut Microbes Lactose Intolerance

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. Gut MicrobesLactose Intolerance

  2. Introduction Our Goals • From last week, we had three possible ways to tackle lactose intolerance: • Convert H2 or methane into less harmful gases • Make a super sugar metabolizing machine • Engineer a strain to NOT convert glucose + galactose into H2 and methane. 2

  3. Goal #2 Super Sugar Metabolizing Machine • In order for our strain of E. coli to consume lactose, we must force it. • In one of our earlier meetings, Doug spoke about his Healthbots, which included the phosphoenolpyruvate:sugar phosphotransferase system (PTS). 3

  4. Goal #2 Super Sugar Metabolizing Machine • To review, some bacteria have a prevelance for certain sugars, mainly glucose, fructose and sucrose (PTS sugars). • To save energy, they will not consume non PTS sugars (lactose, maltose, arabinose), if PTS sugars are present (known as Carbon Catabolite Repression). • We must toggle the PTS by inhibiting the blocking of LacY. 4

  5. Goal #2 Super Sugar Metabolizing Machine 5

  6. Goal #2 Super Sugar Metabolizing Machine • To have our strain uptake lactose, we could introduce specific mutations in LacY. • This will hopefully prevent the binding of EIIA(GLUC), allowing our strain to uptake lactose. • In addition, we can overexpress LacY to help the process. 6

  7. Goal #2 Super Sugar Metabolizing Machine 7

  8. Reference Hoischen C, Levin J, Pitaknarongphorn S, Reizer J, and Saier MH Jr. Involvement of the central loop of the lactose permease of Escherichia coli in its allosteric regulation by the glucose-specific enzyme IIA of the phosphoenolpyruvate-dependent phosphotransferase system. J Bacteriol 1996 Oct; 178(20) 6082-6. pmid:8830713. PubMed HubMed [Hoischen1996] Deutscher, J., Francke, C. and Postma, P.W. 2006. How Phosphotransferase System-Related Protein Phosphorylation Regulates Carbohydrate Metabolism in Bactertia. Microbial and Molecular Biology Reviews. 2006: 939-1031

More Related