1 / 2

Think Outside the Cell

Display-O-Matic. Think Outside the Cell. Display-O-matic. Passenger: Type III Needle scFv. TypeIII Needle scFv: detection of and probiotic for shigella typeIII needle complex Functional Assay: Incubate DH10B cells with his-tagged espA Wash and lyse cells

chesmu
Download Presentation

Think Outside the Cell

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. Display-O-Matic Think Outside the Cell

  2. Display-O-matic Passenger: Type III Needle scFv • TypeIII Needle scFv: detection of and probiotic for shigella typeIII needle complex • Functional Assay: • Incubate DH10B cells with his-tagged espA • Wash and lyse cells • Dry lysate on nitrocellulose, block, and stain with anti-his antibody • Negative controls: • Results: • Displayers only: • Displayers + Needle scFv : 1 2 3 4 5 6 7 8 1 2 3 4 5 6 7 8 9 • Dot blot of …legend • ehaB, VtaA11, OprF, eCPX, yuaQ, azo1653, CPG_L2, espP(beta), AIDA-1

More Related