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Noncanonical Wnt Signaling through G Protein-Linked PKC d Activation Promotes Bone Formation

Noncanonical Wnt Signaling through G Protein-Linked PKC d Activation Promotes Bone Formation. Xiaolin et al. Dev cell . (2007). Formation of the skeleton through intramembranous and endochondral ossification. Intramembranous ossification. Endochondral ossification.

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Noncanonical Wnt Signaling through G Protein-Linked PKC d Activation Promotes Bone Formation

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  1. Noncanonical Wnt Signaling through G Protein-Linked PKCd Activation Promotes Bone Formation Xiaolin et al. Dev cell. (2007)

  2. Formation of the skeleton through intramembranous and endochondral ossification Intramembranous ossification Endochondral ossification Christine Hartmann. Trends cell biol. (2006)

  3. Role of canonical Wnt-b-cat signaling during lineage differentiation and maturation of osteoblasts Christine Hartmann. Trends cell biol. (2006)

  4. Skeletal defects caused by mutations with regard to components of the canonical Wnt pathway Christine Hartmann. Trends cell biol. (2006)

  5. G-protein signaling and PLC mediated PKC activation Classic PKC (cPKC) : a, b1, b2, g → activated by DAG, phosphatidylserine and Ca2+ Novel PKC (nPKC) : d, e, h, q → activated by DAG and phosphatidylserine Atypical PKC (aPKC) : l, i, z → activated by phosphatidylserine Malbon CC. Nat Rev Mol Cell Biol . 2005. Rhee SG. Annu Rev Biochem. 2001.

  6. Penzo-Mendez et al. Dev Biol. (2003) Sheldahl et al. Curr Biol. (1999) Sheldahl et al. J Cell Biol. (2003) Wnts activate PKC through G-protein signal

  7. nPKC Wnt3a osteoblastogenesis Wnt3a induces osteoblast differentiation and MARCKS phosphorylation via PKCd in ST2 cells

  8. PKCd Is Required for Wnt3a-Induced Osteoblastogenesis, but Not for Canonical Wnt Signaling in ST2 Cells aPKC : PKCz pseudosubstrate PKCq pseudosubstrate nPKC(d,q) cPKC Ca2+ PKC

  9. Wnt3a PKCd b-cat osteoblastogenesis Rottlerin Inhibits Wnt3a-Induced Osteoblast Differentiation in ST2 Cells

  10. Wnt3a Gaq Dvl b-cat PLC PKCd osteoblastogenesis PKCd Activation via Gq Signaling Promotes Wnt3a-Induced Osteoblastogenesis and Requires Dvl in ST2 Cells Pertussis toxin : Gi-Gbg inhibitor Gq family : Gq, G11, G14/15/16

  11. PKCd Activation by Wnt3a Correlates with Dvl-2 Translocation to the Plasma Membrane and Is Insensitive to Dkk1 in ST2Cells

  12. Wnt3a Induces Colocalization of PKCδ and Dvl-1,3 at the Plasma Membrane in ST2 Cells

  13. Removal of PKCd Results in a Deficit in Embryonic Bone Formation

  14. Wnt7b Induces Osteoblastogenesis and PKCd Activity, but Does Not Activate Canonical Wnt Signaling

  15. Removal of Wnt7b in Skeletal Cells Results in Defects in Bone Formation

  16. Wnt LRP FZ γ α Dvl β ?? PLC PKCd b-cat Osteoblastogenesis Model for Wnt-PKCd signaling promotes bone formation

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