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Interaction of LSPR with thrombin as an Inhibitor of Blood Clotting

Interaction of LSPR with thrombin as an Inhibitor of Blood Clotting. Brandon T. Brown and Toni A. Trumbo Department of Chemistry and Biochemistry, Bloomsburg University of P ennsylvania, Bloomsburg PA 17815. Blood Clotting. Thrombin Catalytic Site. Vitamin K Antagonists.

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Interaction of LSPR with thrombin as an Inhibitor of Blood Clotting

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  1. Interaction of LSPR with thrombin as an Inhibitor of Blood Clotting Brandon T. Brown and Toni A. Trumbo Department of Chemistry and Biochemistry, Bloomsburg University of Pennsylvania, Bloomsburg PA 17815

  2. Blood Clotting

  3. Thrombin Catalytic Site

  4. Vitamin K Antagonists • Warfarin= most commonly used • “Gold Standard” • Oral available • One dose daily • Short comings • Self-testing • Risk of hemorrhage • Interactions

  5. Direct Thrombin Inhibitors • Dabigatran • Oral available • Less testing • Fewer life-threatening bleeds • Two doses daily • Risks • Harder to reverse or completely irreversible

  6. Peptide table

  7. LSPR • Inhibit thrombin directly • Reversible • Orally available

  8. Clot Assay • 5 µM Fibrinogen was added to Assay buffer • Equilibrated to Room Temperature for 5 minutes • 2.2 nM Thrombin was added, the solution mixed • The solution was inserted into the instrument which then measured the absorbance • 7200 sec • 325 nm

  9. Representative Uninhibited spectrum Hill Equation F=(a(x^b))/((c^b)+(x^b))

  10. Average spectrum and error bars 5 µM LSPR

  11. Comparative plot

  12. Coefficients

  13. Conclusion • LSPR does inhibit clot formation • Maximal inhibition found

  14. Future work • HPLC • Minimum dosage

  15. Acknowledgements • Elizabeth Ola • Jackie North • Department of Chemistry and Biochemistry

  16. References

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