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X-ray Crystallography

X-ray Crystallography. Electron Density Resolution. Nuclear magnetic resonance (NMR). NMR Distance Restraints. Algorithms. Cryo Electron Microscopy ( Cryo -EM). Hydrogen Deuterium (HD) Exchange Mass Spectrometry. Solvent accessible parts of a protein.

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X-ray Crystallography

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  1. X-ray Crystallography

  2. Electron Density Resolution

  3. Nuclear magnetic resonance (NMR)

  4. NMR Distance Restraints Algorithms

  5. CryoElectron Microscopy (Cryo-EM)

  6. Hydrogen Deuterium (HD) Exchange Mass Spectrometry Solvent accessible parts of a protein. D2O is where the hydrogen is replaced by an isotope.

  7. Complex Isotope Patterns in HDX Mass Spectra

  8. CX-MS AP-MS Native MS

  9. Cross Link Known Proteins see if you get false positives in an unrelated databaseUse LDA to compute a combined score

  10. Apply to all proteins in E. coli

  11. Ion Mobility (Mass Spectrometry) Separate by charge and shape of the ion (collisional cross section). Drift time is in milliseconds. Used to detect drugs and explosives. Recently coupled to mass spectrometry

  12. Sugar ions have different shapes.

  13. Protein has multiple conformations (shapes)

  14. Use like LC-MS

  15. From: Ion mobility–mass spectrometry for structural proteomics Expert Rev. Proteomics 9(1), 47–58 (2012)

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