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Moscow Timiryazev Agricultural Academy Department of Agricultural Biotechnology

4. FOOD QUALITY AND SAFETY. Moscow Timiryazev Agricultural Academy Department of Agricultural Biotechnology. FOOD QUALITY AND SAFETY. Department of Agricultural Biotechnology established in 19 86. FOOD QUALITY AND SAFETY. FUNDAMENTAL AND APPLIED RESEARCH:

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Moscow Timiryazev Agricultural Academy Department of Agricultural Biotechnology

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  1. 4 FOOD QUALITY AND SAFETY Moscow Timiryazev Agricultural Academy Department of Agricultural Biotechnology

  2. FOOD QUALITY AND SAFETY Department of Agricultural Biotechnology established in 1986

  3. FOOD QUALITY AND SAFETY • FUNDAMENTAL AND APPLIED RESEARCH: • Marker-assisted selection (MAS) – Use of molecular markers in plant breeding. • Genetic fingerprinting of crop plants • DNA-based diagnostics of hybrid purity and genetic variation of commercial hybrids F1 • DNA-based diagnostics of safety and quality of agricultural products

  4. FUNDAMENTAL AND APPLIED RESEARCH: • DNA-based detection and identification of plant pathogens • Molecular cytogenetics • Genetic engineering • Plant tissue culture – Rapid multiplication, in vitro production of disease-free material, Embryo rescue, microspore culture • Application of plant growth regulators FOOD QUALITY AND SAFETY

  5. FOOD QUALITY AND SAFETY Projects National level Identification of disease resistance genes and creation of the transgenic plants resistant to phytopathogenes – Russian Ministry of Education and Science; Russian Ministry of Agriculture International collaboration New approach for effective analysis, genetic mapping, transformation and testing of agricultural plants genomes -Russian Ministry of Education and Science; The Netherlands, Plant Research International, Wageningen

  6. FOOD QUALITY AND SAFETY • International collaboration • Comparative genome organization of the A.cepa, A.roley, A.fistulosum and tomato. - The Netherlands, Plant Research International, Wageningen University & Research Center • Molecular-cytogenetics of sex determination in Hop. –Germany,Institute ofBiotechnology, University ofHohenheim,Stuttgart;USA, Oregon State University, Corvallis. • Genetic engineering - Australia

  7. FOOD QUALITY AND SAFETY • Department of Agricultural Biotechnology • Results of research studies were published in Russian and international journals – “Plant physiology”, “Genetics”, «Plant Journal», «Genome», «Euphytica» and others.

  8. FOOD QUALITY AND SAFETY Molecular markers allow to reduce a duration of selection process at least twice PCR-RFLPmarker linked to nematode resistance gene Mi S –sensitive plants R – resistant plants

  9. FOOD QUALITY AND SAFETY Cloning of resistance-gene analogs (RGA)

  10. FOOD QUALITY AND SAFETY Development of the molecular marker-based genetic linkage maps

  11. Molecular-cytogenetics of the sex determination in Hop FOOD QUALITY AND SAFETY Y X X : Y = 10 : 5.2 Wild male H. lupulus from Moscow region, Russia. Open bivalent of X and Y chromosomes marked with arrows.

  12. Molecular-cytogenetics of the sex determination in Hop FOOD QUALITY AND SAFETY clone clone M male female M male female 542bp 387bp

  13. FOOD QUALITY AND SAFETY Molecular cytogenetics Genome in situ hybridization (GISH) LILIUM TOMATO TRITICALE

  14. FOOD QUALITY AND SAFETY FOOD QUALITY AND SAFETY Molecular cytogenetics Fluorescence in situ hybridization (FISH)

  15. FOOD QUALITY AND SAFETY Fields of possible collaboration Molecular genetics and cytogenetics Creation of the transgenic plants Genomics of crop plants Bioinformatics

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