1 / 12

Course Snapshot: 680:390 General Microbiology (Lab)

Course Snapshot: 680:390 General Microbiology (Lab). Course Overview. 160 to 170 students each semester Team taught course – Lecture and Lab are separate Lab = 12 weeks 8 section with 20 to 22 students Seating arrangement in lab:. Student Learning Objectives.

crescent
Download Presentation

Course Snapshot: 680:390 General Microbiology (Lab)

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. Course Snapshot: 680:390 General Microbiology (Lab)

  2. Course Overview • 160 to 170 students each semester • Team taught course – Lecture and Lab are separate • Lab = 12 weeks • 8 section with 20 to 22 students • Seating arrangement in lab:

  3. Student Learning Objectives After this unit, students should be able to… …describe the practical applications of serial dilutions. …determine the number of microorganisms in a given culture. …perform a serial dilution and evaluate results.

  4. Alignment Grid

  5. Lab - Week 4/5 Tuesday Pre-lab quiz (10 mins) Mini Lecture (10 mins) discussing their homework and introduce dilutions Introduce case study and have students work in groups of 2 on their dilution schemes. (30 mins) Students prepare their dilutions (45 mins) Tuesday Pre-Lab Quiz Mini Lecture (5 mins) to review what was done previous week Students will get their plates back, count the colonies, and decide whether their dilution scheme was appropriate. (15 mins) Students will write a post-lab which will include purpose, methods, results, discussion and conclusion (15 mins) Wed – Monday Pre-Lab Homework What is a dilution? List a few examples in which you find dilutions in your daily life. Answer the following MC questions. Wed – Monday Post-Lab Homework Students will be presented with another case study for which they have to design an appropriate dilution scheme. Wed – Monday Post-Lab Homework Students will solve a set of dilution questions.

  6. Pre-Lab Quiz Week 4 1. Give a brief definition of a dilution, serial dilution, and a dilution factor. 2. Describe two examples in your daily life in which you would use dilutions. 3. Fill in the dilution factor for each of the following:

  7. In Class Case Study“Would you Swim in Passion Puddle?” You love Passion Puddle, the serene and picturesque little pond in the center of Cook Campus. You have always wondered whether you could safely swim in this pond. Now that you are taking microbiology, you would like to design an experiment and test the water. How would you determine how many organisms are in the water? How would it affect your health if you were to swim in Passion Puddle? The “safe” amount of coliform bacteria is a recommended 126 cells/100mL and the criteria for enterococci for fresh and marine recreation waters is 33 cells/100mL in freshwater and 35 cells/100mL in marine water.

  8. Follow Up Case Study (HW)“What is Cleaner?” One of your fellow classmates got sick after drinking coca cola (with ice) from a soda fountain at a local fast food restaurant. He claims that toilet water is cleaner than the ice cubes that are dispensed from the machine at the restaurant. 1. What organisms do you think causes contamination of ice dispensers? Toilet water? 2. How could you test the water and conclude with water is “cleaner”? Show your work by create an appropriate dilution schemes.

  9. Pre-Lab Quiz Week #5 You perform a serial dilution of a water sample. You pipetted 0.1 mL of sample into 9.9 mL of buffer, mixed the contents and followed with two more 10-2 dilutions. After incubation, you examine your three pour plates. You find the plate counts of the plates are 140, 398 and 563 respectively. • Show your dilution scheme. • What is the viable count (cells/ml) in the original culture?

  10. Survey – Self Assessment • I can identify some practical applications of dilutions. • I know how to identify a dilution factor. • I can create a meaningful dilution scheme. • I can solve a dilution problem.

  11. Final Exam Question You are given a bacterial culture and asked to determine the population (cells/ml). The OD660 = 0.735. For the culture you are testing an OD660 of 1.0 corresponds to approximately 1 X 107cell/ml. Based on the optical density, estimate the population size. Draw a dilution scheme you could use to make pour plates (as you did in lab) to more accurately determine the population size. (Use the back of the page to show your work if necessary.)

More Related