1 / 1

Supplemental Data Figure 3

Supplemental Data Figure 3. PCSK9 WT V5 – Fluor 594. Fluor 488 + 594 + DAPI. PCSK9 WT His – Fluor 488. a. b. c. PCSK9 QH V5 – Fluor 594. Fluor 488 + 594 + DAPI. PCSK9 WT His – Fluor 488. d. e. f.

damian
Download Presentation

Supplemental Data Figure 3

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. Supplemental Data Figure 3 PCSK9 WT V5 – Fluor 594 Fluor 488 + 594 + DAPI PCSK9 WT His – Fluor 488 a b c PCSK9 QH V5 – Fluor 594 Fluor 488 + 594 + DAPI PCSK9 WT His – Fluor 488 d e f Immunocytochemistry showing co-localization (panels c and f) of PCSK9 WT His (using anti-His antibody and Alexa Fluor 488 (Invitrogen)) (panels a and d) with either WT V5 (anti-V5 antibody and Alexa Fluor 594 (Invitrogen)) (panel b) or QH V5 (anti-V5 antibody Fluor 594 (Invitrogen)) (panel e). The table below shows the number of cells that are singly and doubly stained and in brackets the percentage of cells co-expressing both forms of WT PCSK9 or WT and Q152H PCSK9 from five different views. Immunocytochemistry was carried out by sequential double staining protocol as described at http://www.ihcworld.com/_protocols/general_IHC/immunofl_double_squential.htm

More Related