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6 15mu EM big view

(Davi Bock and Clay Reid). 40,000x40,000 pixels 1.6 GB 120x120 m (3 nm/pixel) Here shown 40x undersampled or 1/1600 th of the data in a single section. The goal, 10,000 such sections making up a 3D volume. Then zoom in. Reconstruct the big axons first: the “wires”.

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6 15mu EM big view

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  1. (Davi Bock and Clay Reid) 40,000x40,000 pixels 1.6 GB 120x120 m (3 nm/pixel) Here shown 40x undersampled or 1/1600th of the data in a single section. The goal, 10,000 such sections making up a 3D volume Then zoom in Reconstruct the big axons first: the “wires” Then find the big synapses: the connections 6 15mu EM big view

  2. Andzoom in Find the presynapticterminals 5 8mu rlp

  3. Andzoom in 4 3mu rlp

  4. Andzoom in 3 1mu rlp

  5. Zoomed in ~40x, or 1/1600th of the total field of view. Displayed at native resolution. 2 300 nm rlp Until you identify a synapse (one connection out of many thousand in the circuit)

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