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Percobaan 8 INDUKSI β-GALAKTOSIDASE

Percobaan 8 INDUKSI β-GALAKTOSIDASE. M ichelle (10509016) S urya (10509002) G aby (10509028). Media Pertumbuhan. A (NB) B (NB + glukosa) C (NB + glukosa + laktosa) D (NB + laktosa) E (NB + maltosa) F (NB + galaktosa ). Data Pengamatan. Data Absorbansi. Pengolahan Data.

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Percobaan 8 INDUKSI β-GALAKTOSIDASE

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  1. Percobaan 8INDUKSI β-GALAKTOSIDASE Michelle (10509016) Surya (10509002) Gaby (10509028)

  2. Media Pertumbuhan • A (NB) • B (NB + glukosa) • C (NB + glukosa + laktosa) • D (NB + laktosa) • E (NB + maltosa) • F (NB + galaktosa)

  3. Data Pengamatan

  4. Data Absorbansi

  5. Pengolahan Data • Volume kultur = 1,5 mL • OD600= 0,6 • contoh: pada t=10”

  6. KurvaAbsorbansivs Waktu

  7. KurvaAktivitasvs Waktu

  8. Pembahasan Tabung A (NB) aktivitas β-galaktosidasecukuptinggi tidak ada ATP tidakadainduser Tabung B (NB+glukosa) aktivitas β-galaktosidasesangatsedikit ada sangatbanyakATP tidakadainduser

  9. Pembahasan (lanjutan) Tabung C(NB + glu + lak) aktivitas β-galaktosidasesangatsedikit ada sangatbanyakATP adainduser Tabung D (NB+laktosa) aktivitas β-galaktosidasecukuptinggi adasedikitATP adainduser

  10. Pembahasan (lanjutan) Tabung E (NB +maltosa) adasedikitaktivitas β-galaktosidase ada sedikitATP tidakadainduser Tabung F (NB+galaktosa) aktivitas β-galaktosidasecukuptinggi ada cukupbanyakATP adainduser

  11. Kesimpulan • Tabung D menunjukkanaktivitas β-galaktosidase yang paling tinggi. • Tabung A dan F menunjukkanaktivitas β-galaktosidase yang cukuptinggi. • Tabung E menunjukkansedikit aktivitas β-galaktosidase. • Tabung B dan C menunjukkanaktivitas β-galaktosidase yang paling rendah.

  12. Daftar Pustaka • Berg, Jeremy M., Tymoczko, John L., Stryer, Lubert. “Biochemistry”, 6th ed. 2006. W. H. Freeman and Company. (page 892-901) • Lehninger, Albert L., Nelson, David Lee., Cox, Michael M. “Lehninger Principles of Biochemistry”, 4th ed. 2004. W. H. Freeman and Company. (page 1081-1088) • http://en.wikipedia.org/wiki/Beta-galactosidase (17-04-2012) • http://en.wikipedia.org/wiki/Operon(17-04-2012) • http://en.wikipedia.org/wiki/Lac_operon(17-04-2012)

  13. Terimakasih

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