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NEF

A. B. 3kb gp160 patient env. BamH1. ENV. patient env. Xho1. Kpn1. Stu1. Kpn1. LTR. rev. Bbs1. Sal1. LTR. ori. Xho1. env. pSVIII. pCR. 3.1-. Uni. 7.4. kb. 5.0. kb. SV40 ori. Amp r. Xba1/EcoR1. EcoR1. C. VPR. VPU. TAT. VIF. NEF. REV. GAG. 5’ LTR. POL. ENV.

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NEF

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  1. A B 3kb gp160 patient env BamH1 ENV patient env Xho1 Kpn1 Stu1 Kpn1 LTR rev Bbs1 Sal1 LTR ori Xho1 env pSVIII pCR 3.1- Uni 7.4 kb 5.0 kb SV40 ori Ampr Xba1/EcoR1 EcoR1 C VPR VPU TAT VIF NEF REV GAG 5’ LTR POL ENV 3’ LTR Premature stop codon Supplemental Figure 1A-C.

  2. D NP2/CD4 NP2/CCR5 NP2/CD4/CCR5 env 1 env 2 without AZT with AZT E NP2/CD4/CCR5 Macrophages Supplemental Figure 1D-E.

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