Supplementary figure S1

Supplementary figure S1 Normoxia PBS Hypoxia antagomiR-20a Hypoxia antagomiR_MM Hypoxia PBS Normoxia PBS Hypoxia antagomiR-20a Hypoxia antagomiR_MM Hypoxia PBS 01 02 04 13 14 20 03 26 27 05 06 12 21 22 28 29 09 17 18 19 25 30 10 11 WB: BMPR2 WB: α-tubulin Hypoxia Antago- miR_MM Hypoxia antago- miR-20a Hypoxia PBS Normoxia PBS 07 08 15 16 31 32 23 24 WB: BMPR2 WB: α-tubulin

Supplementary figure S2

Supplementary figure S3 Normoxia PBS Hypoxia antagomiR-20a Hypoxia antagomiR_MM Hypoxia PBS Normoxia PBS Hypoxia antagomiR-20a Hypoxia antagomiR_MM Hypoxia PBS 01 02 04 13 14 20 03 26 27 05 06 12 21 22 28 29 09 17 18 19 25 30 10 11 WB: Id-2 WB: α-tubulin Hypoxia antago- miR_MM Hypoxia antago- miR-20a ns Hypoxia PBS Normoxia PBS * 07 08 15 16 31 32 23 24 WB: Id-2 WB: α-tubulin

3‘UTR CDS miR-20a binding site Supplementary figure S4 72h 48h 24h A B 20a MM 20a MM 20a MM ** WB: Smad5 ** p < 0.01 WB: α-tubulin C Reporter gene assay: 3’UTR of Smad5

Supplementary figure S5 A antagomiR_MM antagomiR-20a WB: Smad5-P WB: Smad5 WB: alpha Tubulin - - 15‘ 15‘ BMP-2 30‘ 60‘ 30‘ 60‘

Supplementary figure S6

Supplementary figure S7 A B C

Supplementary figure S1