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Supplementary figure S1. Normoxia PBS. Hypoxia antagomiR-20a. Hypoxia antagomiR_MM. Hypoxia PBS. Normoxia PBS. Hypoxia antagomiR-20a. Hypoxia antagomiR_MM. Hypoxia PBS. 01. 02. 04. 13. 14. 20. 03. 26. 27. 05. 06. 12. 21. 22. 28. 29. 09. 17. 18. 19. 25. 30. 10.
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Supplementary figure S1 Normoxia PBS Hypoxia antagomiR-20a Hypoxia antagomiR_MM Hypoxia PBS Normoxia PBS Hypoxia antagomiR-20a Hypoxia antagomiR_MM Hypoxia PBS 01 02 04 13 14 20 03 26 27 05 06 12 21 22 28 29 09 17 18 19 25 30 10 11 WB: BMPR2 WB: α-tubulin Hypoxia Antago- miR_MM Hypoxia antago- miR-20a Hypoxia PBS Normoxia PBS 07 08 15 16 31 32 23 24 WB: BMPR2 WB: α-tubulin
Supplementary figure S3 Normoxia PBS Hypoxia antagomiR-20a Hypoxia antagomiR_MM Hypoxia PBS Normoxia PBS Hypoxia antagomiR-20a Hypoxia antagomiR_MM Hypoxia PBS 01 02 04 13 14 20 03 26 27 05 06 12 21 22 28 29 09 17 18 19 25 30 10 11 WB: Id-2 WB: α-tubulin Hypoxia antago- miR_MM Hypoxia antago- miR-20a ns Hypoxia PBS Normoxia PBS * 07 08 15 16 31 32 23 24 WB: Id-2 WB: α-tubulin
3‘UTR CDS miR-20a binding site Supplementary figure S4 72h 48h 24h A B 20a MM 20a MM 20a MM ** WB: Smad5 ** p < 0.01 WB: α-tubulin C Reporter gene assay: 3’UTR of Smad5
Supplementary figure S5 A antagomiR_MM antagomiR-20a WB: Smad5-P WB: Smad5 WB: alpha Tubulin - - 15‘ 15‘ BMP-2 30‘ 60‘ 30‘ 60‘
Supplementary figure S7 A B C