NIAID Strategic Plan

1. New England Regional Center of Excellence for Biodefense and Emerging Infectious Diseases Research NERCE / BEID www.nerce.med.harvard.edu

2. NIAID Strategic Plan • Category A, B, and C Priority Pathogens • Biology of the Microbe • Host Response • Diagnostics • Vaccines • Therapeutics • Research Resources • Regional Centers of Excellence • National Biocontainment Laboratories • Regional Biocontainment Laboratories NERCE / BEID www.nerce.med.harvard.edu

3. NIAID Priority Pathogens NERCE / BEID www.nerce.med.harvard.edu

4. Academic / Industry Partnership NERCE / BEID www.nerce.med.harvard.edu

5. New England Regional Center of Excellence • All medical schools in New England contribute to governance and strategic planning • Institutions currently with active major research projects: • Boston University Medical Center • Center for Blood Research • Harvard Medical School • University of Massachusetts – Dartmouth • University of Massachusetts Medical School • Brigham and Woman’s Hospital • Dartmouth Medical School NERCE / BEID www.nerce.med.harvard.edu

6. NERCE Strategic Plan -NERCE will provide the nation, as quickly and efficiently as possible, with vaccine and therapeutic interventions against infections caused by organisms used as weapons of bioterrorism -NERCE is using a genomics, proteomics, and high-throughput approach to biodefense development

7. NERCE Components • 6 Research Programs • 5 Developmental Projects Program • 4 Core Laboratories / Programs • 2 Career Development Programs • Emergency Response Plan • Regional and National Governance Committees NERCE / BEID www.nerce.med.harvard.edu

8. NERCE Research Programs • Multiple Approaches to Blocking Toxin Action • Direct Inhibition of Anthrax Toxin Action John Collier, PI • Direct Blocking of Botulinium Toxin Action Bal Ram Singh, PI • Disruption by Small Molecules of Intracellular Toxin Transport Tomas Kirchhausen, PI • Mechanism(s) of Endocytic Vesicle Membrane Translocation by Diptheria Toxin, Anthrax Toxin, and Botulinium Neurotoxins Jack Murphy, PI NERCE / BEID www.nerce.med.harvard.edu

9. NERCE Research Programs • Mediators and Inhibitors of Immunity to Yersinia pestis Michael Starnbach and John Goguen, PIs • Microbial Vectors for Antigen Delivery John Mekalanos, David Knipe, and Darren Higgins, PIs • Development of a Conjugate Vaccine for the Prevention of Tularemia Arthur Tzianabos and Dennis Kasper, PIs NERCE / BEID www.nerce.med.harvard.edu

10. NERCE Research Programs • Discovery of Small Molecules to Block Fusion by Envelope Proteins of Dengue and Ebola Viruses Stephen Harrison, PI • Innate Immunity to Hemorrhagic Fever Viruses Robert Finberg and Douglas Golenbock, PIs NERCE / BEID www.nerce.med.harvard.edu

11. NERCE Developmental Projects • Studies of the SARS Receptor ACE2 Michael R. Frazan, PI • Structure of Anthrax Spore Polysaccharide Julia Wang, PI • SARS Coronavirus Pathogenesis in African Green Monkeys Keith G. Mansfield, PI • Development of a Multi-gene Subunit-based smallpox vaccine Shan Lu, PI • Novel Ways to Optimize CTL Response to Vaccinia Virus William R. Green, PI NERCE / BEID www.nerce.med.harvard.edu

12. NERCE Core Laboratories • BSL-3 Animal and Tissue Culture Andrew Onderdonk, PI • Proteomics Leonardo Brizuela, PI Harvard Institute of Proteomics • Small Molecule Screening Steven Lory, PI Institute for Chemistry and Cell Biology • Clinical Studies Raphael Dolin, PI NERCE / BEID www.nerce.med.harvard.edu

13. Career Development Program Career Development Program RFP Issued October 14 Applications due December 1 Funding Start March 1 NERCE / BEID www.nerce.med.harvard.edu

14. Emergency Response Plan • Provide facilities and scientific support to first-line responders in the event of a biodefense or naturally occurring biological emergency • Independent local confirmatory testing of a positive test result derived from Project BioWatch • Scientific expert resource • Laboratory testing consulting services, auxiliary sample testing, and sample storage and archiving NERCE / BEID www.nerce.med.harvard.edu

15. Regional Centers of Excellence • Duke University • Harvard Medical School • New York State Department of Health • University of Chicago • University of Maryland • University of Texas Medical Branch • University of Washington • Washington University in St. Louis NERCE / BEID www.nerce.med.harvard.edu

16. NBLs and RBLs • National Biocontainment Laboratories • Boston University • University of Texas Medical Branch • Regional Biocontainment Laboratories • Colorado State University • Duke University • Tulane University • University of Alabama at Birmingham • University of Chicago • University of Medicine and Dentistry of New Jersey • University of Missouri • University of Pittsburgh • University of Tennessee

17. Assembly and Action of Anthrax Toxin NERCE / BEID www.nerce.med.harvard.edu

18. Screening for chemicals that protect cells from anthrax toxin Cell Viability NERCE / BEID www.nerce.med.harvard.edu