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ISTA GMO Task Force Objectives and Progress 2001-2002 Enrico Noli

ISTA GMO Task Force Objectives and Progress 2001-2002 Enrico Noli. The ISTA GMO Task Force. Chair: Dr. Enrico Noli IT Vice-chair: Mr. Alexander B. Ednie CA Members:. Dr. Eugenia Barros ZA Mrs. Sofia Ben Tahar FR Mr. Eric Gatignol FR Mr. Leopold Girsch AT Mr. Sylvain Grégoire FR

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ISTA GMO Task Force Objectives and Progress 2001-2002 Enrico Noli

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  1. ISTA GMO Task Force Objectives and Progress 2001-2002 Enrico Noli

  2. The ISTA GMO Task Force • Chair: Dr. Enrico NoliIT • Vice-chair: Mr. Alexander B. EdnieCA • Members: Dr. Eugenia BarrosZA Mrs. Sofia Ben TaharFR Mr. Eric GatignolFR Mr. Leopold GirschAT Mr. Sylvain GrégoireFR Dr. Lutz GrohmannDE Mr. Tim GutormsonUS Dr. Christoph HaldemannCH Mrs. Adelaida HarriesAR Mr. Benjamin KaufmanUS Mr. Nigel KilbyFL Mrs. Berta KillermannDE Dr. Michael KruseDE Dr. Bernard Le BuanecCH Prof. Dr. Norbert LeistDE Dr. Anja MatzkDE Mr. Richard C. PayneUS Mr. Chris SikolasGR Mr. Marc van den BulckeBE Mr. David Zhang FR

  3. ISTA´s Vision • ISTA seed testing laboratories are competence centres for • varietal purity testing of GM varieties • detection, identification and quantification of GM seeds in conventional seed lots • Uniformity in GMO seed testing results all over the world

  4. 2. Organisation of Proficiency Tests • PILOT ISTA GMO PROFICIENCY TEST • AIMS • to evaluate the performance of participating laboratories • to collect data to be used to set performance standards • to gain experience in the organisation of future tests

  5. PILOT ISTA GMO PROFICIENCY TEST • PRELIMINARY WORK • Statistical work carried out by the STA-GMOTF (S. Gregoire, K. Remund and J.L. Laffont) • Effect of reference material seed impurity influence • Effect of false positive/negative rate on consumer and producer risk • Number of samples to be tested by each lab for a sufficiently precise evaluation of its proficiency is quite high (several test rounds will be necessary)

  6. PILOT ISTA GMO PROFICIENCY TEST • TEST PROTOCOL • Species: Zea mays • Number of samples: 30 (some positive,some negatives) • Seeds/sample: 300 • Potentially present events: Bt176, Bt11, Mon810, T25 • Type of result: qualitative • No prescribed or suggested analytical procedure • MATERIAL TRANSFER AGREEMENT (MTA)

  7. PILOT ISTA GMO PROFICIENCY TEST • PURITY TEST of the NON-GM SEED • 30,000 seeds analysed • 15 samples, 2000 seeds/sample • 2 DNA extraction/sample • Targets: • - P-35S real time PCR (LOD=0.02%) (K. Haldemann) • - T-NOS qualitative PCR (LOD=0.02%) (D. Zhang) • Results: all samples tested negative • The level of GMO adventitious presence < 0.01% (P 0,05) • Test was organised and started on May 24 from the ISTA Secretariat (B.Kahlert, M. Kruse)

  8. 3. Platform for exchange of information “ISTA/FAO Workshop on Electrophoretic and PCR-based methods for varietal verification and GMO detection” Buenos Aires, 3-7 December 2001 18 participants from South America Theoretical part: basic elements on PCR, GMO detection methods Practical part: DNA extraction, control of DNA quality,qualitative PCR detection methods for GMOs

  9. FUTURE OBJECTIVES • Continuing the draft of the Rules Chapter and Annexes • Evaluation of the results of the first ISTA GMO proficiency test and utilisation of the data obtained to define performance standards • Continuing the collaboration with the STA and BSC Committees for the design of future tests • Consolidating and extending the proficiency test program – next round by end 2002 • The ISTA homepage as well as the ISTA Web Board will be organised as a platform for discussion • Establishing a data base with information about testing methods, validation projects and links • Organizing other Workshops specifically aimed at GMO analysis

  10. Preliminary draft for the second ISTA GMO PROFICIENCY TEST • TEST PROTOCOL • Species: Glycine max • Events: RR soybean (Monsanto) • Number of samples: 5 • Seeds/sample: 3000 • Type of result: qualitative and quantitative threshold (1%) • No prescribed or suggested analytical procedure • Participation fee required for non-members

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