LEUKOCYTE EVALUATION

1. LEUKOCYTE EVALUATION Clinical Textbook for Veterinary Technicians 4th edition Dennis M. McCurninSuanders

2. WBCs are categorized as 1) granulocytes (neutrophils, eosinophils, basophils) * characterized by segmented (lobed) nuclei and expect for the neutrophil, distinct cytoplamsmic granules Or 2) agranulocytes (lymphocytes, monocytes) * also referred to as monocuclear cells and do not have segmented nuclei

4. Neutrophils: the predominant WBC * phagocytic * bacteriocidal * important in inflammatory conditions The average time spent by a neutrophil in the blood is about 10 hours. Normal neutrophils have deeply staining, clumped, segmented nuclei with relatively clear cytoplasm (sometimes a faint dusting of granules)

5. Segmented Neutrophils

6. Morphological changes in the neutrophil: Bands – appearance of band shaped nuclei indicated the release of immature neutrophils Even more immature neutrophils have oval or bean shaped nuclei.

7. Neutrophils may show signs of inflammatory disease as demonstrated by certain cytoplasmic characteristics: • Toxic neutrophils – • Dohle bodies – small, pale bluish-gray irregular inclusions in the cytoplasm that usually indicate mild toxemia.

8. Generalized basophilia of the cytoplasm or cytoplasmicvacuolation are slightly more severe toxic changes.

9. Neutrophilia – increased number of total neutrophils. Neutropenia – a decrease in circulating neutrophils. May occur when tissue demand is excessive as a result of severe inflammation exceeding the ability of the bone marrow to supply the cells.

10. Eosinophils * help control allergic or anaphylactic hypersensitivity reactions The eosinophil is characterized by a segmented nucleus, colorless to pale-blue cytoplasm and distinct eosinophilic (reddish-orange) staining granules in the cytoplasm

11. Eosinophils Per species: Cats – contain numerous tiny rod-shaped granules that may obscure the nucleus Dogs – granules are less numerous and usually round but may vary considerably in size. Horses – granules are extremely distinctive, being very large and round and a much brighter orange than smaller animals Bovine – granules are also bright orange but are much smaller and more numerous than those of the horse.

12. Basophils Relatively rare in blood smears. When they do appear they tend to occur in association with increased eosinophils. They have dark basophilic (blue) granules.

13. Basophils per species: Cats – granules tend to be light lavender to almost pink Dogs – have few to no granules and must be differentiated from neutrophils. Equine and Bovine – tend to have variable numbers of more typical dark granules

14. Lymphocytes Usually small to medium sized mononuclear cells with a thin rim of light to dark-blue cytoplasm and a round nucleus. The cytoplasm may or may not contain red granules.

15. Monocytes • Derived from bone marrow • Circulate in the blood briefly before entering the tissues in which they become macrophages • Phagocytize large particles and cellular debris that neutrophils cannot handle. Monoctyes have gray-blue, often grainy cytoplasm and a variable shaped nucleus.