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Lab Meeting 10 /25/ 2013

Lab Meeting 10 /25/ 2013. Catherine Doyle. TET + 1mMCaffeine. TET + 1mM Theophylline. TET. c.Dog RBS. eCDM8. tetA. psB1A8. psB1A2. tetA. c.Dog RBS. eCDM8. psB1A8. GGA Results. MW. 1 2 3 4 5 6 7 8 9 10 C1 C2. Insert bp = 4100kb. Steps/Progress.

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Lab Meeting 10 /25/ 2013

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  1. Lab Meeting 10/25/2013 Catherine Doyle

  2. TET + 1mMCaffeine TET + 1mM Theophylline TET

  3. c.Dog RBS eCDM8 tetA psB1A8 psB1A2 tetA c.Dog RBS eCDM8 psB1A8

  4. GGA Results MW 1 2 3 4 5 6 7 8 9 10 C1 C2 Insert bp = 4100kb

  5. Steps/Progress • Move tetA module to psb1A8 • iPCR • GGA • Colony PCR • Verify through tetA resistance and PCR

  6. Next Steps • Verify Insert • Finish program

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