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Lab meeting

Lab meeting. 2014.02.24 이세라. Gene Scan –B2M. PCR. electrophoresis. Sample 2. Sample 1. 95℃ / 5min. Sequencing-PLK1. Childhood AML -RNA. cDNA 합성. 65 ℃ / 5min -> ice. 2 5 ℃ / 10min -> 50 ℃ / 50min ->85 ℃ / 5min -> ice. 1 μ l of Rnase H. 37 ℃ / 20min -> ice. cDNA ->electrophoresis.

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Lab meeting

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  1. Lab meeting 2014.02.24 이세라

  2. Gene Scan –B2M PCR

  3. electrophoresis Sample 2. Sample 1. 95℃ / 5min

  4. Sequencing-PLK1 Childhood AML -RNA

  5. cDNA합성 65 ℃ / 5min -> ice 25 ℃ / 10min -> 50 ℃ / 50min ->85 ℃ / 5min -> ice 1μl of Rnase H 37 ℃ / 20min -> ice

  6. cDNA->electrophoresis cDNA PCR->electrophoresis

  7. PLK1 -Primer

  8. pyrosequencing 2014.02.24(BDNF) 2013.09.24(SLC6A4) PCR전 PCR후

  9. flowcytomety 2014.02.21 M-MDSC G-MDSC

  10. 2/20sample 조일도71411924 M-MDSC G-MDSC

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