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Family Enterobacteriaceae Genus

Enterobacteria. Biological characteristic and classification of genera. Characteristics of genus Escherichia, Shigella and Salmonella. Escherichia Shigella Edwardsiella Salmonella Citrobacter Klebsiella. Enterobacter Hafnia Serratia Proteus Morganella Providencia Yersinia Ervinia.

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Family Enterobacteriaceae Genus

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  1. Enterobacteria. Biological characteristic and classification of genera. Characteristics of genus Escherichia, Shigella and Salmonella

  2. Escherichia Shigella Edwardsiella Salmonella Citrobacter Klebsiella Enterobacter Hafnia Serratia Proteus Morganella Providencia Yersinia Ervinia Family EnterobacteriaceaeGenus

  3. Escherichia

  4. Antigenic structure • O antigen • K antigen • H-antigen These antigens detected by agglutination reactions.

  5. Biochemical reactions • They ferment most of the sugars • Glucose • Lactose • Mannitol • Maltose with production acid and gas. • Indolreaction is positive. • H2Sis not formed. • Gelatin is not liquified.

  6. Diarrhoea • E.coli causing diarrheal diseases are of five groups. 1. EnteropathogenicE.coli (EPEC) 2. EnterotoxigenicE.coli (ETEC) 3.Enteroinvasive E.coli (EIEC) 4.Enterohaemorrhagic E.coli (EHEC) 5.Enteroaggregative E.coli (EAEC)

  7. Laboratory diagnosis • Bacteriological method.

  8. Shigella • Classification • Subgroup A (S.dysenteriae) • Subgroup B (S.flexneri) • Subgroup C (S.boydii) • Subgroup D (S.sonnei)

  9. Morphology

  10. Laboratory diagnosis • Bacteriological method.

  11. Salmonella • Classification • Causative agents of enteric fever S.typhi , S.paratyphi A, S.schottmuelleri • Causative agents of gastroenteritis S.typhimurium, S.enteritidis, S.newport, S.dublin and etc. 3. Salmonella causing septicemia S.cholerae-suis

  12. Morphology of salmonellae

  13. Antigenic structure • Flagellae antigen – H • Somatic antigen – O • Surface antigen – Vi, found in some species.

  14. ClassificationKauffmann-White scheme • Salmonellae are classified into groups based on distinctive O antigen factors. • Within each group, further identification and differentiation is by phase 1 and phase 2 H antigens.

  15. Culture • Lactose-including media • SS-agar • Nutrient agar • Bile broth • Wilson and Blair bismuth sulphite medium (BSA)

  16. Biochemical reactions • Salmonellae ferment: • Glucose • Mannitol • Maltose forming acid and gas except S.typhi which produces only acid and no gas. • Most salmonellae produce H2S. • They do not ferment lactose. • Indol is not produced.

  17. Laboratory diagnosis • Isolation of bacilli • Demonstration of antibodies • Demonstration of circulating antigen

  18. Vaccination • TAB vaccine – • Live oral typhoid vaccine • Purified Vi polysaccharide vaccine

  19. Salmonella typhi

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