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CULTURE COLLEGE. Presented by: Celeste Industries Corporation. BIOLOGICAL OPPORTUNITIES for CLEANING with CELESTE BIOZYME. Drain Maintenance. Lavatory Odor and Mildew Control. Biozyme II T reated. Biozyme II Treated. Biozyme II Mildewstat Property. Long Term Carpet Odor Control.
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CULTURE COLLEGE Presented by: Celeste Industries Corporation
Biozyme II Treated Biozyme II Treated Biozyme II Mildewstat Property
Airline Opportunities for Biozyme • Lavatories • Toilet shroud cleaning and odor control • Floors • Drain maintenance • Cabin • Carpet cleaning additive for odor control • Galley • Drain & Drain Mast maintenance Celeste Biozyme provides a bio-film that continues to work after your employees are finished cleaning.
BASIC MICROBIOLOGY • How does Biozyme work? • Enzyme function. • Microbial life cycle
Bacteria – Enzyme Relationship ENZYMES BACTERIUM BACTERIA, which are living single celled organisms, produce ENZYMES, which are non-living molecules.
Enzymes • Non-living molecules produced by bacteria • Biochemical catalysts • Break down organic waste • Specific activity
Protein Lipid (fat) Starch Protease Enzyme Activity Enzyme Substrates
Enzyme Activity Protease and Starch Protease and Lipid (fat) Protease and Protein
Enzyme Activity Protease and Protein Lipase and Lipid (fat) Amylase and Starch
Enzymes and Their Substrates • Protease – protein • Urease – urea • Amylase – starch • Cellulase – cellulose • Lipase – fat • Xylanase – plant material • Esterase – fat
Protease Lipase Esterase Cellulase Xylanase Amylase
Naturally Occurring Bacteria • Single celled, living organisms • Found virtually everywhere • Consume organic waste • Prolific enzyme production
Requirements for Bacterial Activity • Water • Nutrients (organic waste) • Time • Correct environmental conditions
Essential Bacterial Profile • Reproduce quickly • Safe, non-pathogenic, naturally occurring • Form spores for long term stability • High level production of multiple enzymes • Function in or under diverse environmental conditions
BACILLUS The type of bacteria used in Celeste Biozyme
Bacillus Vegetative Cells • Enzyme producers • Consume organic waste • Grow logarithmically • Mobile • Form spores when conditions dictate dormancy is appropriate
Bacillus Spores (Dormant Cells) • Heat resistant • Chemical resistant • Desiccation resistant • UV resistant • Germinate to vegetative cells when environmental conditions are right
Spore Formation VEGETATIVE CELL VEGETATIVE CELL WITH SPORE Spore Germination Vegetative Cell Lysis FREE SPORE Bacillus Lifecycle
H2O CO2 Bacillus Waste Digestion Analogy Bacterium Enzymes Bacterium digests waste and release CO2 and H2O Enzymes break down waste into small pieces Pizza (organic waste)
Growth Curve Population Time
Bacterial Respiration • Aerobic - Oxygen required • Anaerobic - Oxygen not required • Facultative Anaerobic - Grows with or without oxygen Celeste Biozyme is functional with or without oxygen.
Functional Temperature and pH Ranges • 41° - 131° F (5° - 55° C) • pH 4.5 - 10 Celeste Biozyme is functional in a wide range of temperature and pH