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5.2. PARAMETERS. NQAD GAS: Air FILTER: 1.3 sec GAIN: 1X EVAPORATION TEMP: 35C ELSD GAS: Nitrogen, 1.5 L/min GAIN: 1 EVAPORATION TEMP: 40C HPLC MOBILE PHASE: 40.60 ACN:Water with 0.2% TFA. APPLICATION NOTES. 4.3. 3.0. 0.00. 1.00. 2.00. 4.4. mV. NQAD , 0.64 ng S/N = 15.
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5.2 PARAMETERS NQAD GAS: Air FILTER: 1.3 sec GAIN: 1X EVAPORATION TEMP: 35C ELSD GAS: Nitrogen, 1.5 L/min GAIN: 1 EVAPORATION TEMP: 40C HPLC MOBILE PHASE: 40.60 ACN:Water with 0.2% TFA APPLICATION NOTES 4.3 3.0 0.00 1.00 2.00 4.4 mV NQAD, 0.64 ng S/N = 15 FLOW RATE: 1.00 mL/min COLUMN: Phenomenex Kinetex C18, 150 x 4.6, 2.6 u COLUMN TEMP: Ambient INJECTION VOLUME :1 µL CONCENTRATION: 8 ng and 0.64 ng on column ANALYTE: Prednisone ™ QUANT TECHNOLOGIES, LLC 1463 94TH Lane NE Blaine, MN, USA 55449 763-398-0508 www.Quant-NQAD.com 4.4 0.00 1.00 2.00 3.0 SUB NANOGRAM SENSITIVITY CAN BE ACHIEVED WITH THE NQAD, ENSURING ALL SAMPLE COMPONENTS CAN BE DETECTED Application note # QAN-0088 CHROMATOGRAMS NQAD, 8 ng S/N = 240 ELSD, 8 ng S/N = 8 Note: This application was run at Quant Technologies on a single ELSD and may not be representative of all ELSD’s.