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LIFE 12 ENV IT 000652 Cleansed

Explore the sediment samples from Canale dei Navicelli using Optical and Atomic Force Microscopy to analyze shape, size, granulometry, and biological composition. Use high-resolution optical microscopy and AFM methods with specialized software for quantitative analysis. Understand the physical and biological properties of soil by classifying particles based on diameter.

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LIFE 12 ENV IT 000652 Cleansed

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  1. Optical and Atomic Force Microscopy for analysis of wetsedimentsfrom the Canale dei Navicelli LIFE 12 ENV IT 000652 Cleansed

  2. Analysis with Optical Microscopy (shape, size, granulometry) • Analysis with scanning probe by AFM (Atomic Force Microscope) Twomethods

  3. Biologicalmicroscope with high-resolution(16 Mpixel) reflex on trinocular tube for digitalimaging Software: ImageJ(developed by the National Institute of Health): for quantitative analysis (realdimensions-pixel, particlecounting) HeliconFocus Pro: for image stacking (to increase the depth of field) Optical Microscopy

  4. NanosurfEasyscan 2 Microscope • Scanning head: 110*110 micron • Maximum Z range: 22 micron • Maximum resolution: 2048*2048 pixel AFM Microscopy

  5. The texture has great importance for the physical and biological properties of the soil • Classification (USDA): • clay, diameter less than 2 micron • silt, diameter between 2 and 50 micron • sand, between 50 micron and 2 mm. Granulometry

  6. Sample 1 Total counting: 4002 Sample 3 Total counting: 2005

  7. Sample 5 Total counting: 2013 Sample 7 Total counting: 3442

  8. Sample 1 Cyclotella Meneghiniana (MC) Cyclotella Meneghiniana (Moder. Cont.) Staurastrum anatinum (MC)

  9. Sample 1 Cyclotella Meneghiniana (MC) Melosira Varians (MC) Melosira Moniliformis (MC) Navicula Rhyncotella (MC)

  10. Sample 1 Cyclotella Meneghiniana (MC) Melosira Moniliformis (MC) Cyclotella Meneghiniana (MC) Bacillaria Paxillifer (MC to HC)

  11. Sample 1 AFM measurements of sedimentary rock composed by centric

  12. Sample 1 AFM: trace of diatom (Nitzschia Acicularis (MC to HC))

  13. Sample 3 Surirella Brebissonii (MC) Nitschia Sublinearis (MC to HC) Gyrosigma Nodiferum (MC)

  14. Sample 3 AFM: trace of diatom (Phase contrast)

  15. Sample 3 AFM: trace of diatom

  16. Sample 3 AFM: trace of diatom Zoom at 45 micron

  17. Sample 5

  18. Sample 7 Fragilaria Acus (SC) Fragilaria Acus (SC)

  19. Sample 7 In some cases the AFM cantilever can displace a diatom: Before: After:

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