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ABO & Rh Blood Typing Lab. Name in MLA Format Title Read the introduction and write a 5 sentence introduction summary for the lab. Hypothesis – based on your family genetics what blood type do you think you are? Read and write down the procedures so you know what to do Wednesday
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Name in MLA Format • Title • Read the introduction and write a 5 sentence introduction summary for the lab. • Hypothesis – based on your family genetics what blood type do you think you are? • Read and write down the procedures so you know what to do Wednesday • Draw Tables 1 and 2. For table 1 increase the tables to include 4 blood samples (1 for each person in your group) • Write down the following assessment questions leaving room to answer each question: • 1 – 9, 11-12 • Conclusion – be sure to address whether your hypothesis was right or wrong, what you learned, etc. Lab Write Up Procedures
Put on all lab safety equipment. • Wash your hands with soap and water. • Thoroughly clean the tip of one finger on your non-writing hand with a sterile alcohol pad. • Carefully remove a lancet package from the end that is closest to the blunt end of the lancet and then remove it. • Prick the sterile area on your finger with the lancet. • If you cannot perform this step, ask your teacher for help. • Carefully place the lancet back in its package and dispose of it in the biohazard bag. Procedures
Add one drop of blood to each well of the blood typing tray. • Make sure that you work quickly and there is enough blood that it does not dry to the tray. • Clean the tip of your finger with a new sterile alcohol pad and dispose of it in the biohazard bag. • Add one drop of anti-A serum to the A well, one drop of the anti-B serum to the B well, and one drop of anti-Rh to the Rh well. • Using a clean toothpick, stir the A well thoroughly. Dispose of the toothpick in the biohazard bag.
Repeat step 10 for B and Rh wells. Be sure to use a new toothpick for each well to avoid cross contamination. • Examine each well for agglutination. Agglutination indicates a positive test result. • Record your results in Table 1 and determine your blood type. • Pool the class data and calculate the percentage of students with each blood type using the following formulas: Total number of students with type (x) blood X 100 Total number of students in class • Record your results in Table 2.