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LSRCP Production Meeting 2011. Re: RY2010 N atural origin adult summer Chinook collection for integrated brood stock production for U.S. v. Oregon. ADULT COLLECTION : HOW TO? LOGISTICS & COORDINATION.
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LSRCP Production Meeting 2011 • Re:RY2010 Natural origin adult summer Chinook collection for integrated brood stock production for U.S. v. Oregon
ADULT COLLECTION : HOW TO? LOGISTICS & COORDINATION • Collection issues & concepts for capturing natural origin fish (NOR) from the south fork salmon river for IBS production up to 250K smolt • Collect & pond NOR adults @ weir; ISS impact • Collect M & F NOR fish below weir on spawning grounds & transport to holding ponds; practicality & logistics issues • Collect milt from NOR males during trapping and cross with hatchery females • Collect milt from ripe NOR males day of and day before egg take • Coordination & Record Keeping • IDFG Research (Laurie Janssen & crew) and NPT (Rob Hill & Carl East) assisted with data recording (trapping db) and milt collection • Used genetic ID to track male milt to spawn x w/ hatchery female
TECHNIQUES & METHODS • Same day & day before spawn take milt collection • Ripe NOR male milt collected, fish passed above weir • Whirl Pac bags were used for collection & labeled w/ genetic ID number • Milt placed in coolers and/or refrigerated if collected the day before egg take • Foot pump was used to add air to the bags to allow the sperm to respire • Paper towel was used to dry the area around the vent • Each NOR male crossed with a single hatchery female • Incubated in trays as singles • No IBS eggs were culled due to high BKD; 381K green collected
SPAWN DATA & EYED EGG RESULTS • 77 NOR males (x w/ 72 hatchery females) used out of 347 trapped during spawning operations • Natural origin males w/ PITs (7) • 3 “Wild” & 4 “Unknown” origin • Same day vs. day before egg take milt collection: IBS eye % • Same day = 88 % • Day before = 69 % • Overall IBS eye % vs. overall hatchery prod eye % • Overall IBS eye % = 77.5 % • Overall hatchery production eye % = 90 %
IBS Prod Numbers • 264,000 BY2010 IBS fry on station • Approx 22K excess IBS production mixed w/ 70K hatchery production • All IBS production will be marked as “unclipped CWT only”