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Morphologic and molecular pathogenesis study of lesions in condemned porcine kidneys

Morphologic and molecular pathogenesis study of lesions in condemned porcine kidneys . Claudia Benavente MSc Student cebenave@ucalgary.ca . Outline. Background Objective of the study Materials and Methods Results Summary Pending studies. Background. 1,490. 2,550. 3,900. 2,735.

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Morphologic and molecular pathogenesis study of lesions in condemned porcine kidneys

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  1. Morphologic and molecular pathogenesis study of lesions in condemned porcine kidneys Claudia Benavente MSc Student cebenave@ucalgary.ca

  2. Outline • Background • Objective of the study • Materials and Methods • Results • Summary • Pending studies

  3. Background 1,490 2,550 3,900 2,735 www.scholastic.ca

  4. Healthy pigs www.usda.gov. Less economic impact with condemnation of carcasses or organs at the slaughterhouse Less potential public health implications (Zoonosis)

  5. Condemned pig kidneys Petechiae Cyst Tumors “White-spotted”

  6. Objective of the study • Identify microorganisms involved in the pathogenesis of the condemned kidneys with “white-spots” lesions in market hogs of a slaughterhouse near Calgary, Alberta. • Bacteriological culture, histochemistry, polymerase chain reaction (PCR test), Western blot for serological test.

  7. Materials and Methods • Sample collection • Condemned and normal • Tattoo number • Serum • Frozen tissue • Histology • Swabs • Bacteriological study • Mass-spectrometry

  8. Histoquemistry. • Gram stain • Ziehl-Neelsen stain • Grocott’sMethenamine silver stain (GMS) • Periodic acid-Schiff stain (PAS) • Warthin- Starry stain

  9. Macroscopic classification Grade 0 Grade 1 Grade 2 Grade 3

  10. Microscopic Classification

  11. Severe histological lesions

  12. ResultsRelationship between Macroscopic and Histological classification

  13. Results

  14. Results • Six out of ten control kidneys were positive to bacteria isolation, bacteria were grown in 23 /30 (76%) condemned kidneys. • Acinetobacter spp., Streptococcus spp. and Stenotrophomonasmaltophiliawere the bacteria most frequently isolated. • It was found that 28/29(96%) isolated bacteria were grown in Thioglycollate broth, 16/29(55%) in Sheep Blood Agar, and 8/29(28%) in McConkey agar. • There was no growth of any bacteria in anaerobic conditions. • Microorganisms (bacteria, fungi, Mycobacterium or leptospires) were not detected in tissue using histochemistry.

  15. Summary • Actinobacillusequuliand A. suiswere not isolated in this study, and lesions compatible with infection by these organisms were not observed. • Different degrees of microscopic lesions were detected in condemned kidneys, independent of the macroscopic grade classification (size and distribution of the lesions). • Acinetobacterlwoffi, A. parvushave been recently associated with serious infection in human patients with broad antimicrobial drug resistance (Turton et al.,2010). • Stenotrophomonasmaltophiliais an important nosocomial pathogen and clinicians should not underestimate the significance of this bacterium infection (Falagas et al., 2009; Araoka et al., 2010).

  16. Pending studies • Polymerase Chain Reaction (PCR) test for detection of porcine parvovirus, porcine circovirus type 2, porcine reproductive and respiratory syndrome virus in tissue. • Western blot test to determine presence of host’s immune response to microorganisms detected. • Statistical analysis of the data.

  17. Acknowledgements • Supervisor: Dr. Carmen Fuentealba, UCVM & Faculty of Medicine • Committee members: Dr. Glen Armstrong, Faculty of Medicine Dr. Rebekah De Vinney, Faculty of Medicine Dr. Anne Muckle Dr. Marcus Czub Lisa Ashton Maureen Bukhari Ramona Taylor Jan Giles Lorraine Lund • Funding: UCVM Fellowship Research grant: Department of Ecosystem and Public Health

  18. Any comments or suggestions? www.elated.co

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