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Spektroskopi Konvensional

SPEKTROFOTOMETRI 1.Ultra Violet ( λ 200-380 nm) 2. Visible ( λ 380-700 nm) 3. Fluorometri 4. Serapan Atom. Spektroskopi Konvensional. 1. Sumber Radiasi. Argon 100 – 160 nm Tungsten 350 – 800 nm Deuterium 160 – 360 nm Xenon 200 – 900 nm. 2. Kuvet (Sample Container).

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Spektroskopi Konvensional

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  1. SPEKTROFOTOMETRI1.Ultra Violet (λ 200-380 nm) 2. Visible (λ 380-700 nm)3. Fluorometri4. Serapan Atom

  2. Spektroskopi Konvensional

  3. 1. Sumber Radiasi • Argon 100 – 160 nm • Tungsten 350 – 800 nm • Deuterium 160 – 360 nm • Xenon 200 – 900 nm

  4. 2. Kuvet (Sample Container)

  5. 3. Monokromator PRISMA

  6. GRATING

  7. 4. Detektor Photovoltaic Phototube Diode array

  8. T = I/I0 A = Log 1/T A = a . b. c a = k.p.x • T = Transmiten • Io = Intensitasdatang • I = “ sinarygditransmisikan k = tetapan =8,7 . 1019 • A = Absorbansi p = probabilitas (0-1) • a = absorbtivitas molar (L mol-1 cm-1) x = area molekul (10 Ao • b = panjanglart yang dilaluisinar (cm) (panjangkromopor) • c = konsentrasi (mol/L)

  9. TAHAPAN MELAKUKAN ANALISIS DENGAN SPEKTROFOTOMETRI 1. Apakahsenyawatersebutdapatdianalisis denganspektrofotometri 2. Membuatlarutanstandar 3. Menentukanlarutanpanjanggelombang maksimum 4. MembuatkurvabakuantaraAbsorbansi versus konsentrasisehinggadiperoleh persamaanregresi linier. 5. Menentukankadarsampel.

  10. 1. Membuattarutanstandarparasetamol 100 ppm sebanyak 100 ml : ditimbangparasetamolmurni 10 mg laluditambahetanolabsolutsampai 100 ml 2. Membuatserilarutanstandarmisal 10 ppm, 20 ppm 30 ppm, 40 ppmdan 50 ppm 3. Mencaripanjanggelombangmaksimum 4. MembuatkurvakalibrasiAbsobansi VS Konsentrasi 5. Membuatpersamaanregrasilininier Y = a + bx

  11. Y = a + bx Y = Absorbansi a = intersep (titikpotonggarisregresi terhadapsumbu y, a = + titikpotong diatastitik 0,0 , a = - titikpotong dibawahtitik 0,0) b = slope (kemiringangaris / tgsudut ) x = Konsentrasilarutanstandar/sampel

  12. r =

  13. ContohSoal 100 mg sampel yang mengandungParasetamoldilarutkandenganetanolhingga 100 ml. diambil 10 ml diencerkanhingga 100 ml. darilarutan yang sudahdilarutkandiukurAbsorbansinya ternyata A = 0,465. hitunglahkadarparesetamoldalamsampeltersebut ?

  14. Dari data diatasdiperoleh a = 0,096 b = 0,013 r = 0,9852 sehinggadiperolehpersamaan y = 0,096 + 0,013 x jika y = 0,465 maka x = 28,38

  15. Kadar = C.reg. x P x V beratsampel = 28,38 mg/L x 10 x 0,1 L x 100 % 100 mg = 28,38 %

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