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Protein Synthesis (Gene Expression) Proteins (Review) Proteins make up all living materials

Protein Synthesis (Gene Expression) Proteins (Review) Proteins make up all living materials. Proteins are composed of amino acids – there are 20 different amino acids Different proteins are made by combining these 20 amino acids in different combinations.

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Protein Synthesis (Gene Expression) Proteins (Review) Proteins make up all living materials

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  1. Protein Synthesis (Gene Expression) Proteins (Review) • Proteins make up all living materials

  2. Proteins are composed of amino acids – there are 20different amino acids • Different proteins are made by combiningthese 20 amino acids in different combinations

  3. Proteins are manufactured (made) by the ribosomes

  4. Function of proteins: • Help fight disease • Build new body tissue • Enzymes used for digestion and other chemical reactions are proteins (Enzymes speed up the rate of a reaction) • 4. Component of all cell membranes

  5. DNA Replication during Mitosis • -DNA replication begins with the "unzipping" of the parent molecule as the hydrogen bondsbetween the base pairs are broken. • Remember A-T and G-C • At the end you have 2 identical strands of DNA- one for the new cell and one for the parent cell • This entire process happens very rapidly and requires the aid of several different enzymes ex. DNA polymerase, helicase……….

  6. Making a Protein—Transcription • First Step:Copying of genetic information from DNA to RNA called Transcription Why? DNA has the genetic code for the protein that needs to be made, but proteins are made by the ribosomes—ribosomes are outside the nucleus in the cytoplasm. DNA is too large to leave the nucleus (double stranded), but RNA can leave the nucleus (single stranded).

  7. Part of DNA temporarily unzips and is used as a template to assemble complementary nucleotides into messenger RNA (mRNA).

  8. mRNA then goes through the pores of the nucleus with the DNA code and attaches to the ribosome.

  9. Making a Protein—Translation • Second Step:Decoding of mRNA into a protein is called Translation. • Transfer RNA (tRNA) carries amino acids from the cytoplasm to the ribosome.

  10. These amino acids come from the food we eat. Proteins we eat are broken down into individual amino acids and then simply rearranged into new proteins according to the needs and directions of our DNA.

  11. A series of three adjacent bases in an mRNA molecule codes for a specific amino acid—called a codon. • A triplet of nucleotides in tRNA that is complementary to the codon in mRNA—called an anticodon. • Each tRNA codes for a different amino acid. Amino acid Anticodon

  12. mRNA carrying the DNA instructions and tRNA carrying amino acids meet in the ribosomes.

  13. Amino acids are joined together to make a protein. Polypeptide = Protein

  14. Use one of the codon charts to find the amino acid sequence coded for by the following mRNA strands. CAG/CCU/GCU/UGA ___________/___________/___________/____________ AAA/UUC/GAU/UAA • ___________/___________/___________/____________ Start vs. stop( termination) codons

  15. 2nd Base 1st Base 3rd Base • CAC/CCA/UGG/UGA • ___________/___________/___________/____________ Histidine Proline Tryptophan Stop

  16. AUG/AAC/GAC/UAA • ___________/___________/___________/____________ Methionine Asparagine Aspartic Acid Stop

  17. Protein Synthesis

  18. http://www.lpscience.fatcow.com/jwanamaker/animations/Protein%20Synthesis.htmlhttp://www.lpscience.fatcow.com/jwanamaker/animations/Protein%20Synthesis.html

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