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Importance of Nutritional and Physical Characteristics of Diets for Mass Rearing Screwworm & Pink Bollworm

Importance of Nutritional and Physical Characteristics of Diets for Mass Rearing Screwworm & Pink Bollworm. Norman C. Leppla, Muhammad F. Chaudhury & Fred D. Stewart University of Florida and USDA. New World Screwworm Cochliomyia hominivorax (Diptera:Calliphoridae). Screwworm Life Cycle.

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Importance of Nutritional and Physical Characteristics of Diets for Mass Rearing Screwworm & Pink Bollworm

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  1. Importance of Nutritional and Physical Characteristics of Diets for Mass Rearing Screwworm & Pink Bollworm Norman C. Leppla, Muhammad F. Chaudhury &Fred D. Stewart University of Florida and USDA

  2. New World ScrewwormCochliomyia hominivorax(Diptera:Calliphoridae)

  3. ScrewwormLife Cycle Adults Eggs on wound Pupae in ground Larvae in wound

  4. Myiasis in Livestock

  5. Screwworm Larval Media Nutria Whale

  6. Screwworm “Factories” Mission Tuxtla Gutierrez

  7. Water Feeder Mixer Ingredient Feeder Diet preparation

  8. Pumping diet into trays

  9. Tray with larval diet

  10. Larvae falling from trays

  11. Screwworm pupae

  12. Cotton Linter Rearing System

  13. Composition of Larval Diet Spray-dried bovine blood 6% Spray-dried egg 4% Milk substitute 4% Gelling agent (or 6% Cellulose) 2% Water (or 79.9% with cellulose) 83.9% Formalin 0.1%

  14. Spray Dried Whole Bovine Blood • Protein 85% • Fat 1% • Ash 4.5% • Fiber 1% • Moisture 6%

  15. Egg Powder • Protein 50-58% • Fat 25-30% • Moisture 5% or less • Cholesterol trace • Vitamins A, B1,2,6,12,D,E, Folate, Biotin • Minerals Na, K, Ca, P, Mg, Fe, Cu, Cl, I, Zn

  16. Milk Powder Substitute Protein 20 g Fat 28 g Carbohydrate 42 g Moisture 3 g Ash 3 g Total Vitamins ~1 g A, B1, B2, B3, B6, B12, Biotin C, D, E, Folate, Pantothenic acid Minerals ~2.5 g Calcium, Copper, Iodine, Iron Magnesium, Phosphorous Potassium, Sodium, Zinc

  17. Physical Characteristics • Role of gelling agent (or cellulose fiber) - Provide texture and matrix to diet - Gel polymer is not bio-degradable - Cellulose is biodegradable • Importance of viscosity - Larval mobility and growth - Proper functioning of equipment • Importance of pH -pH change occurs during feeding - Feeding not effected between 6.8 & 5.5

  18. Pink BollwormPectinophora gossypiella(Lepidoptera: Gelechiidae)

  19. Pink Bollworm Life Cycle

  20. Pink Bollworm Rearing Facility

  21. Diet Preparation

  22. Diet Extrusion

  23. Larval Development and Cutout

  24. Collection of Pupae

  25. Larval Diet Composition • Wheat germ • Sugar • Soy flour • Yeast • Anti-microbials (3) • Vitamins(8) • Choline chloride • Red dye

  26. Successful Diet Characteristics • Stimulate and enable feeding • Contain essential nutrients • Organize ingredients in a matrix • Nutrients biologically available

  27. Successful Diet Characteristics • Chemically stable & clean • Eliminate anti-nutrients • Easy to prepare • Easy to discard (biodegradable) • Provides a “happy” home

  28. For more information or a copy of this presentation please visit: http://ipm.ifas.ufl.edu

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