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4 TH ANNUAL ADVISORY PANEL MEEETING

This presentation provides an overview of the RosBREED project, its mission to develop marker-assisted breeding to enhance the efficiency of rosaceous cultivar release and adoption, and its focus on fruit quality. It also discusses the project's target outcomes, management structure, and collaboration with industry stakeholders.

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4 TH ANNUAL ADVISORY PANEL MEEETING

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  1. 4TH ANNUALADVISORY PANEL MEEETING • 10 January 2013 • San Diego, California

  2. Outline of Presentation • Project Overview • Morning Session • Afternoon Session RosBREED

  3. Project Overview

  4. RosBREED Mission Statement We will develop and apply marker-assisted breeding, based on improved knowledge of industry value and consumer preferences, to accelerate and increase the efficiency of rosaceous cultivar release and successful cultivar adoption Amy Iezzoni, Michigan State Univ. Cameron Peace, Wash. State Univ. 4 yrs $7.2M federal $7.2M matching This project is supported by the Specialty Crop Research Initiative of USDA’s National Institute of Food and Agriculture RosBREED

  5. RosBREEDWillBridgetheChasm Genomics knowledge Marker- assisted breeding Breeding Genomics Programs Research Genomics Resources More efficient development of new cultivars

  6. RosBREED’s Focus is on Fruit Quality

  7. RosBREED Will Develop Tools For those traits that have value for producers, market intermediaries and consumers $ Marker-assisted breeding in application

  8. RosBREED Will Use Improved Knowledge • of industry values and consumer preferences to select target traits for Marker-Assisted Breeding • Impacts: increase new cultivar adoption • enlarge market potential Courtesy David Byrne, Texas A&M Univ.)

  9. Supporting Breeding Decisions Which traits to target? Which parents to use? Which combinations to create? Which seedlings to progress? Which selections to trial? WA2 Which advanced selections to commercialize?

  10. Supporting Breeding Decisions Which wild germplasm to use? Which traits to target? Which parents & combinations to create? Which seedlings to progress? Which selections to trial? WA2 Which advanced selections to commercialize?

  11. RosBREED’s Target Outcomes Increased gain in fruit quality per breeding cycle due to improved parent selection and improved mean progeny value Long-term outcomes Four year project outcomes Implementation of MAB by breeding programs More rapid availability of new cultivars with genetically superior fruit quality Improved profitability and sustainability of US rosaceous fruit, nut, and floral crops with increased consumption and enjoyment RosBREED

  12. RosBREED’s Management • Executive Committee • Nahla Bassil • Michael Coe • Gennaro Fazio • Karina Gallardo • Ksenija Gasic • Jim Luby • Dorrie Main • Jim McFerson • Cameron Peace • Eric van de Weg • Cholani Weebadde • Chengyan Yue • Project Director • Amy Iezzoni • Project Assistants • Audrey Sebolt • Joan Schneider Genomics Team Leader: Dorrie Main Extension Team Leader: Cholani Weebadde Breeding Team Leader Jim Luby Genotyping Team Leader: Nahla Bassil • Apple • Jim Luby • Susan Brown • Kate Evans • Peach • Ksenija Gasic • John Clark • Dave Byrne • Tom Gradziel Socio- Economics Team Leader: Chengyan Yue • Strawberry • Chad Finn • Jim Hancock • Tom Davis Pedigree- Based Analysis Team Leader: Eric van de Weg • Cherry • Amy Iezzoni • Nnadozie Oraguzie MAB Pipeline Team Leader BIMS Team Leader: Gennaro Fazio Cameron Peace

  13. Demonstration Breeders & Breeding Trainees

  14. Distribution & Processing • Advisory Panel Members Industry Extension Scientific Production Bierlink Curtis Perry Sparks Seavert Bliss Cook Harrison van Sickle Cheng Simon Hirst Watkins Abbott Buell Gardiner Hummer Allen Goldberger Carr Olmstead Dodd Sherman Grim Arus KorsonAvari Lott Baugher Stokes Ross Karp Moffitt Consumer & Markets

  15. International Collaborators Plant Research International, Netherlands East Malling Research, UK CRA-FRU, Rome, Italy IASMA, Trento, Italy INRA (Bordeaux, Avignon, Angers), France Andres Bello University, Chile Plant & Food Research, New Zealand ARC, South Africa RosBREED participant location + 2 Scientific Advisory Panel members (Spain, New Zealand)

  16. Morning Session Goal = provide information to AP membersto set the stage for receiving advice

  17. Morning Session RosBREED

  18. Major Enabling Deliverables • Enabling computer-based accomplishments that allow us to find, polish and put the Jewels into application • Computer demonstrations at breaks: CholaniWeebadde Jewels in the Genome in eXtension Eric van de Weg Pedigree-Based Analysis software Dorrie Main BIM System

  19. External Evaluation of Extension Efforts • Dr. Michael Coe • Cedar Lake Research Group, LLC

  20. From Natural Diversity to Better Cultivars

  21. Afternoon Session Goal = receive advice from AP members

  22. Afternoon Session • Group 1: Remain in the ballroom • Group 2: Move to Cortez room RosBREED

  23. Discussion Session What methods or plans of work could be done differently to better meet RosBREED’s current industry and breeding project goals?  What unanticipated opportunities, needs and limitations should we address within the current project? Thinking ahead to a second project, how well do our proposed objectives effectively address current and future industry and breeding opportunities, needs and limitations? What modifications would you recommend? RosBREED

  24. Jewels in the Genome Analogy An individual’s genome is the full complement of genetic information that it inherited from its parents. Within this vast repertoire of genetic information, individuals genes are being discovered that control critical production and fruit quality traits. Jewels = valuable genetic discoveries ready for breeding application = marker-locus-trait associations validated in a breeding program Find Polish Put into application in breeding

  25. RosBREED seeks to find, polish and put into application predictive DNA tests for valuable Jewels in the Genome

  26. “Jewels in the Genome” Available Now!

  27. The Jewels are Linked on Chromosomes 56 chromosomes in strawberry!!

  28. Acknowledgements This project is supported by the Specialty Crop Research Initiative of USDA’s National Institute of Food and Agriculture

  29. Cornell Susan Brown KenongXu Clemson KsenijaGasic Gregory Reighard Texas A&M Dave Byrne Univ. of CA-Davis Tom Gradziel Carlos Crisosto Univ. of New Hamp. Tom Davis MSU Amy Iezzoni (PD) Jim Hancock Dechun Wang CholaniWeebadde WSU Cameron Peace Dorrie Main Kate Evans Karina Gallardo Vicki McCracken Nnadozie Oraguzie Former WSU Raymond Jussaume Mykel Taylor Univ. of Arkansas John Clark USDA-ARS Nahla Bassil Gennaro Fazio Chad Finn Univ. of Minnesota Jim Luby Chengyan Yue Oregon State Univ. Alexandra Stone Plant Research Intl, Netherlands Eric van de Weg Marco Bink

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