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Viry studované v Laboratoři strukturní virologie : pikornaviry včelí viry Leishmania RNA virus

Proč viry způsobují onemocnění ?. Co jsou viry - jsou živé ?. Metody studia struktury virů : rentgenová krystalografie kryo-elektronová mikroskopie. Viry studované v Laboratoři strukturní virologie : pikornaviry včelí viry Leishmania RNA virus bakteriofág 812K1. Infekce chřipkou.

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Viry studované v Laboratoři strukturní virologie : pikornaviry včelí viry Leishmania RNA virus

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  1. Pročviryzpůsobujíonemocnění? Co jsouviry - jsouživé? • Metodystudiastrukturyvirů: • rentgenovákrystalografie • kryo-elektronovámikroskopie • VirystudovanévLaboratořistrukturnívirologie: • pikornaviry • včelíviry • Leishmania RNA virus • bakteriofág 812K1

  2. Infekcechřipkou

  3. Dvěformy existence viru: “Virion” – infekčnívirovápartikule Infekcebuňky

  4. Jsouviryživé?

  5. Cyklusbuněčnéhodělení

  6. dsDNA dsDNA ssRNA Proteiny

  7. Viry Buněčnéorganismy Replikace dsDNA DNA Transkripce ssRNA Proteiny RNA Translace Proteiny

  8. Strukturyvirionů Ikosahedrální Helikální Komplexní Nepravidelné

  9. WILHELM CONRAD RÖNTGEN (1845-1923) • 1901 Nobel Laureate in Physics discovery of the remarkable rays subsequently named after him

  10. MAX VON LAUE (1879-1960) • 1914 Nobel Laureate in Physics for his discovery of the diffraction of X-rays by crystals

  11. Wavelength and diffraction

  12. Wavelength comparison of X-rays and visible light 38l

  13. 70μm

  14. James Batcheller Sumner(1879-1960) • 1946 Nobel Laureate in Chemistry for his discovery that enzymes can be crystallized

  15. FRANCIS HARRY COMPTON CRICK (1916~2004)JAMES DEWEY WATSON (1928~)MAURICE HUGH FREDERICK WILKINS (1916~2004) • 1962 Nobel Laureates in Physiology and Medicine for their discoveries concerning the molecular structure of nuclear acids and its significance for information transfer in living material. James Watson and Francis Crick Rosalind Franklin Maurice Wilkins

  16. Max Ferdinand Perutz (1914 – 2002)John Cowdery Kendrew (1917 – 1997) • 1962 Nobel Laureate in Physics for their studies of the structures of globular proteins

  17. Information from X-ray diffraction experiment y z x [0;0;0]

  18. 1. Virus purification 2. Crystallization 3. Diffraction data 4. Solve structure

  19. 1. Virus purification 2. Grid preparation 3. cryo-EM 4. Reconstruction

  20. Picornaviry Rhinoviry – 40% “nachlazení” – finančnínáklady $16bn/rok v USA Enteroviry (EV71) – nemocrukou, nohou a úst – encefalitida McMinn et al. Clin Infect Dis 2001. Image by Heng Soy, KI Media 2012.

  21. Životnícykluspikornavirů Virus replication factory

  22. Včelíviry

  23. Leishmania RNA virus 1 metastasis to nasopharyngeal tissues Free Dictionary Ives et al. Science, 2011 Olivier. Nature, 2011 cutaneousleishmaniasis

  24. Totiviry - Leishmania RNA virus 1 (LRV1) Fujimura et al. PNAS, 2011 Naitow et al. NSMB 2002

  25. Kryo-EM fága 812 infikujícího S. aureus Daniel Němeček, JiříNováček, LubomírKováčik

  26. Infekcefágem T4

  27. Pročviryzpůsobujíonemocnění? Co jsouviry - jsouživé? • Metodystudiastrukturyvirů: • rentgenovákrystalografie • kryo-elektronovámikroskopie

  28. Picornavirus virion

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