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European project « 5th PCRDT »

European project « 5th PCRDT ». GARLIC AND EALTH Second annual report Partner P8 CIRAD-FLHOR. ADVANCEMENT OF THE Works. Scientific and administrative team. L. FEREOL scientist Duties: somatic embryogenesis, histology and flow cytometry S. CAUSSE technical assistant

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European project « 5th PCRDT »

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  1. European project « 5th PCRDT » • GARLIC AND EALTH • Second annual report • Partner P8 CIRAD-FLHOR

  2. ADVANCEMENT OF THE Works

  3. Scientific and administrative team • L. FEREOL scientist • Duties: somatic embryogenesis, histology and flow cytometry • S. CAUSSE technical assistant • Duties: somatic embryogenesis, maintenance of the material • M. Roux-Cuvelier • Duties: field trials • R. Kahane • Duties: management

  4. Objectives • Somatic embryogenesis • Comparative characterisation of plantlets from somatic embryos

  5. Work plan • Establishment of somatic embryogenesis procedure for four varieties • Rouge Reunion • Messidrome • Morasol • Printanor • Production and provision of somatic embryos via callus and via cell suspensions of these varieties

  6. Work plan (continue) • Genetic evaluation of somatic embryos derived plants by : • flow cytometry (by P8) • biochemistry (by P5) • molecular markers (by P1) • morphological and physiological analyses (by P8, P9)

  7. Provision of 200 plantlets from embryogenic calli « Rouge Reunion » Provision of 200 plantlets from embryogenic cell suspension « Rouge Reunion » Production of embryogenic callus « Messidrome », « Morasol » Initiation of embryogenic cell suspension « Messidrome », « Morasol » Production and germination of callus and cell suspension derived somatic embryos Milestones of the first year (= achieved;  partly achieved)     

  8. Genetic characterisation of somatic embryos derived plantlets « Rouge Reunion » • By flow ctytometry(P8), finger printing(P1), dry matter and sulphur content(P5), morpho-physiology(P9) Provision of 200 plantlets from embryogenic calli of « Messidrome », « Morasol » Provision of 200 plantlets from embryogenic cell suspension of « Messidrome », « Morasol » Genetic characterisation of these plants by flow cytometry Milestones of the second year    

  9. Production of embryogenic callus « Printanor » Initiation of embryogenic cell suspension « Printanor » Production and germination of callus and cell suspension derived somatic embryos « Printanor » Milestones of the second year (continue)   

  10. DP 5 paper on: Evidence of a somaic embryogenesis process and plant regeneration and acclimatisation in garlic (Allium sativum L.) L. FEREOL, V. CHOVELON, S. CAUSSE, N. MICHAUX FERRIERE and R. KAHANE S U B M I Ted DP13 paper on: Establishment of garlic (Allium sativum L.) cell suspensions and plant regeneration L. FEREOL, V. CHOVELON, S. CAUSSE, D. TRIAIRE, N. MICHAUX FERRIERE and R. KAHANE In P R O G R ESS Deliverables

  11. RESEARCH ACTIVITIES

  12. Different morphological and histological aspect of garlic calluses Compact non embryogenic Compact with embryogenic potentiel Semi-friable non embryogenic Semi-friable with embryogenic potentiel Friable non embryogenic Friable with embryogenic potentiel

  13. Established cell suspension Histological aspect Microscopical aspect Macroscopical aspect

  14. Cell suspension before plating Histological aspect Microscopical aspect Macroscopical aspect

  15. Plating after 16 days Histological aspect Macroscopical aspect

  16. Plating after 30 days Histological aspect Macroscopical aspect

  17. Plating after 45 days Histological aspect Macroscopical aspect

  18. Media used for garlic somatic embryogenesis via callus and via cell suspension

  19. Growth of cell suspension cultures

  20. Effect of age of cell suspension culture on its multiplication rate

  21. Effect of age of cell suspension culture on its regeneration

  22. Effect of cell density at plating on number of embryos regenerated

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