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PEG protocol

. Cleaning, 2 hrs. Step A (Slides). Step B (Coverslips). Step C (Reaction containers). . . . Step D, 1hr(Aminosilanization). . Step E, 2hr incubation(PEGylation). Notes. Reaction containers: any glass containers which are used for aminosilanization. We recycle them. We use a couple of glass sta

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PEG protocol

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    1. PEG protocol as of April 2005

    3. Notes Reaction containers: any glass containers which are used for aminosilanization. We recycle them. We use a couple of glass staining dishes and a flask. Aminosilane: obtained from UCT, Cat. # A0700, 25mg, expire after six months PEG: obtained from Nektar Therapeutics. biotin-PEG (0H4M0H02, BIO-NHS-5000), mPEG (2M4M0H01, M-SPA-5000) Methanol: ACS grade The other chemicals such as sodium bicarbonate: from Fisher The diameter of our centrifuge is 5”. The power of our sonicator is 130W.

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