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ARDRA identification scheme of coryneforms

bp. Lanes indicated with M: molecular size markers (100 base pair ladder (Pharmacia Biotech, Uppsala, Sweden). Figure 1. ARDRA patterns obtained after restriction of the amplified 16S rDNA with Cfo I. 1: pattern 1, Corynebacterium afermentans subspecies afermentans (CIP 103499T)

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ARDRA identification scheme of coryneforms

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  1. bp

  2. Lanes indicated with M: molecular size markers (100 base pair ladder (Pharmacia Biotech, Uppsala, Sweden). Figure 1. ARDRA patterns obtained after restriction of the amplified 16S rDNA with CfoI. 1: pattern 1, Corynebacterium afermentans subspecies afermentans (CIP 103499T) 2: pattern 2, CDC coryneform group ANF 1-like (IBS T30014) 3: pattern 3, C. minutissimum (ATCC 10288T) 4: pattern 4, C. urealyticum (ATCC 43042T) 5: pattern 4+, CDC coryneform group ANF 3-like (IBS T88848) 6: pattern 5, C. amycolatum (CIP 103452) 7: pattern 6, C. pseudotuberculosis bv. equi (CIP 5297) 8: pattern 7, CDC coryneform group B-1 (IBS T94631) 9: pattern 8, CDC coryneform group B-3 (IBS T95303) (Pattern 9 (coryneform group B-3 (IBS T79396) : not shown) Figure 2. ARDRA patterns obtained after restriction of the amplified 16S rDNA with AluI. Lane numbers correspond with pattern numbers: 1: Corynebacterium afermentans subspecies afermentans (CIP 103499T) 2: C. afermentans subsp. lipophilum (CIP 103500T) 3: C. diphtheriae (CIP 100721T) 4: C. jeikeium (CIP 103337T) 5: C. pilosum (ATCC 29592T) 6: C. propinquum (CIP 103792T) 7: C. cystitidis (CIP 6940) 8: 'C. macginleyi' (IBS B72932) 9: CDC coryneform group F-1, genomospecies 1 (IBS B1657) 10: C. pseudotuberculosis bv. ovis (CIP 102968T) 11: C. renale (CIP 6937) 12: CDC coryneform group ANF3-like (IBS T88848) 13: CDC coryneform group B-1 (IBS T94631) 14: CDC coryneform group B-3 (IBS T79396) 15: Rhodococcus equi (CIP 5472T). Figure 3. ARDRA patterns obtained after restriction of the amplified 16S rDNA with RsaI. 1: pattern 1, C. afermentans subsp. lipophilum (CIP 103500T) 2: pattern 2a, C. diphtheriae (CIP A 102) 3: pattern 2b, C. pseudotuberculosis bv. equi (CIP 5297) 4: pattern 2c, C. pseudotuberculosis bv. ovis (CIP 102968T) 5: pattern 3, C. propinquum (IBS B50121) 6: pattern 4, C. striatum (CDC E 8873) 7: pattern 5, C. urealyticum (ATCC 43043) 8: pattern 6, C. xerosis (IAM 12431) 9: pattern 7, CDC coryneform group G, genomospecies 1 (LCDC 81613) 10: pattern 8, CDC coryneform group F-1, genomospecies 1 (IBS B1657) 11: pattern 9, CDC coryneform group ANF 3-like (IBS T88848) 12: pattern 10, C. renale (CIP 103421T) 13: pattern 11, CDC coryneform group B-3 (IBS T95303) 14: pattern 12, CDC coryneform group B-3 (IBS T79396) 15: pattern 13, Rhodococcus equi (CIP 5472T)

  3. ARDRA identification scheme of coryneforms

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