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Rhizome Project Meeting(2) 5-4-2011

Rhizome Project Meeting(2) 5-4-2011. Min- Jeong Kim Gang Lab Institute of Biological Chemistry Washington State University Pullman, WA. Why Johnsongrass ?. Johnsongrass. www.usda.gov. Distribution of Johnsongrass. www.usda.gov. Work Flow. Total RNA. cDNA synthesis.

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Rhizome Project Meeting(2) 5-4-2011

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  1. Rhizome Project Meeting(2)5-4-2011 Min-Jeong Kim Gang Lab Institute of Biological Chemistry Washington State University Pullman, WA

  2. Why Johnsongrass?

  3. Johnsongrass www.usda.gov

  4. Distribution of Johnsongrass www.usda.gov

  5. Work Flow Total RNA cDNA synthesis PCR with gene-specific primers LR rxn with pK7GWIWG2(II) Sequence confirmation Cloning into pCR8/GW/TOPO vector Sequence confirmation Transfer into LBA4404 Agro. strain Plant transformation

  6. 0 week 1 week 2 weeks 4 weeks

  7. Expression Analysis ofKnox Transcription Factors(Based on Ginger and Turmeric Microarray Data)

  8. Knox (1) Intensity

  9. Knox (2) Intensity

  10. Knox (3) Intensity

  11. Knox (4) Intensity

  12. Knox (5) Intensity

  13. Alignment of Knox genes in Sorghum bicolor (1)

  14. Alignment of Knox genes in Sorghum bicolor (2)

  15. Alignment of Knox genes in Sorghum bicolor (3)

  16. Alignment of Knox genes in Sorghum bicolor (4)

  17. Map of pK7GWIWG2(II) RNAi Vector Karimi, M., Inzé, D., Depicker, A., Gateway vectors for Agrobacterium-mediated plant transformation. Trends Plant Sci. 2002 May;7(5): 193-195

  18. Transgenic Plants on Selection Media

  19. Transgenic Plants in the Greenhouse

  20. Insert Confirmation (1) M PC NT 2 6 10 M M, marker; PC, positive control (vector); NT, non-transgenic

  21. Insert Confirmation (2) M PC NT 2 6 10 12 16 M NC M, size marker; PC, positive control (vector); NT, non-transgenic; NC, negative control (without PCR template)

  22. Thank You

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