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Two weeks old cereal plants. Incubate plants with Agrobacterium tumefacians culture. Wash plants with sterile water or MS salt solution. Stress treatment. GUS assay. Microscopic analysis of eGFP. Add carbenicilin supplemented MS salt solution and incubate the plants at RT.
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Two weeks old cereal plants Incubate plants with Agrobacterium tumefacians culture Wash plants with sterile water or MS salt solution Stress treatment GUS assay Microscopic analysis of eGFP Add carbenicilin supplemented MS salt solution and incubate the plants at RT