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VETTORI PER TERAPIA GENICA

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VETTORI PER TERAPIA GENICA

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    1. Adenovirus Retrovirus Virus adeno-associati Lentivirus (derivati da HIV) Herpesvirus Plasmidi nudi Elettroporazione in vivo Liposomi Amine, proteine cariche positivamente VETTORI PER TERAPIA GENICA

    2. Classificazione Adenovirus

    3. ADENOVIRUS

    6. Relative size of Adenovirus

    7. TASSONOMIA

    8. ADENOVIRUS STRUCTURE

    10. TUTTI GLI Ad HANNO UNA SIMMETRIA ICOSAEDRICA

    11. ADENOVIRUS INFECTION

    12. ADENOVIRUS ENTRY

    14. Ingresso del virus nella cellula ospite

    15. ADENOVIRUS GENOME

    17. ADENOVIRUS REPLICATION

    19. Ciclo biologico dell’adenovirus

    20. PERCHE’ UTILIZZARE VETTORI ADENOVIRALI?

    21. TIPI DI VETTORI ADENOVIRALI VETTORI ADENOVIRALI DI PRIMA GENERAZIONE (FG-Ad) VETTORI ADENOVIRALI DI SECONDA GENERAZIONE Vettori Adenovirali Helper-dependent

    22. VETTORI ADENOVIRALI

    24.

    27. pAd-Easy System Il gene di interesse: vettore shuttle Cotrasformazione in E. coli. (BJ5183) con pAdEasy-1 Il plasmide linearizzato e trasfettato in cellule permissive 293

    28. VETTORI ADENOVIRALI

    32. Tossicitŕ dei vettori adenovirali

    42. Vettori Ad e Tossicitŕ

    43. Vettori HD-Ad

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