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Safety in Lab and Field Exercises

Safety in Lab and Field Exercises. Biology Department Furman University. Required for completion of the “Lab Safety Acknowledgement Form”. Science is an Adventure. Science is an adventure into the ‘unknown’ and ‘unfamiliar’ Unknown, unfamiliar places. Science is an Adventure.

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Safety in Lab and Field Exercises

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  1. Safety in Lab and Field Exercises Biology Department Furman University Required for completion of the “Lab Safety Acknowledgement Form”

  2. Science is an Adventure • Science is an adventure into the ‘unknown’ and ‘unfamiliar’ • Unknown, unfamiliar places

  3. Science is an Adventure • Science is an adventure into the ‘unknown’ and ‘unfamiliar’ • Unknown, unfamiliar places • Unknown, unfamiliar methods and materials

  4. Science is an Adventure • Science is an adventure into the ‘unknown’ and ‘unfamiliar’ • Unknown, unfamiliar places • Unknown, unfamiliar methods and materials • Unknown, unfamiliar chemical reactions and organismal responses THAT’S WHY IT’S FUN!!!

  5. Travel Safely • Science is an adventure into the ‘unknown’ and ‘unfamiliar’ • To safely enjoy unfamiliar terrain, you should “Shoot PARR”: • Be PREPARED – think ahead, have a plan, know your objectives • Be AWARE of your surroundings • Take RESPONSIBILITY • Follow the RULES

  6. Travel Safely • Science is an adventure into the ‘unknown’ and ‘unfamiliar’ • To safely enjoy unfamiliar terrain, you should “Shoot PARR”: • Be PREPARED – think ahead, have a plan, know your objectives

  7. Travel Safely • Science is an adventure into the ‘unknown’ and ‘unfamiliar’ • To safely enjoy unfamiliar terrain, you should “Shoot PARR”: • Be PREPARED – think ahead, have a plan, know your objectives Before Lab: What will I need for lab? Am I dressed correctly for today’s lab or field exercise? Do I have personal safety items? (epipen, inhaler, glasses vs. contacts)

  8. Travel Safely • Science is an adventure into the ‘unknown’ and ‘unfamiliar’ • To safely enjoy unfamiliar terrain, you should “Shoot PARR”: • Be PREPARED – think ahead, have a plan, know your objectives In Lab: Get ready to work - Get the things you’ll need: pen/pencil, notebook, etc. - Put the other stuff AWAY (bookbagsstowed, food and drink in hall)

  9. Travel Safely • Science is an adventure into the ‘unknown’ and ‘unfamiliar’ • To safely enjoy unfamiliar terrain, you should “Shoot PARR”: • Be PREPARED – think ahead, have a plan, know your objectives Get ready to work: Read the lab handout before doing anything! - What is the objective of this procedure? - What steps are necessary to achieve this objective? - What materials, are needed for each step? Where are they? - When should the steps be taken, and how quickly?

  10. Travel Safely • Science is an adventure into the ‘unknown’ and ‘unfamiliar’ • To safely enjoy unfamiliar terrain, you should “Shoot PARR”: • Be PREPARED – think ahead, have a plan, know your objectives Get ready to work: Read the lab handout before doing anything! Examine each apparatus/tool/reagent/organism before using it! - What is the correct way to use it? - Where is the on/off switch? (How would you turn it off in an emergency?) - What does each dial or indicator do? - Is there anything potentially dangerous about it?

  11. Travel Safely • Science is an adventure into the ‘unknown’ and ‘unfamiliar’ • To safely enjoy unfamiliar terrain, you should “Shoot PARR”: • Be PREPARED – think ahead, have a plan, know your objectives • Be AWARE of your surroundings

  12. Travel Safely • Science is an adventure into the ‘unknown’ and ‘unfamiliar’ • To safely enjoy unfamiliar terrain, you should “Shoot PARR”: • Be PREPARED – think ahead, have a plan, know your objectives • Be AWARE of your surroundings Locate exits (How do I get out, or back to the van?)

  13. Travel Safely • Science is an adventure into the ‘unknown’ and ‘unfamiliar’ • To safely enjoy unfamiliar terrain, you should “Shoot PARR”: • Be PREPARED – think ahead, have a plan, know your objectives • Be AWARE of your surroundings Locate exits (How do I get out of the room or back to the van?) Locate safety equipment (eye-wash, first-aid kit, extinguisher, etc.)

  14. Travel Safely • Science is an adventure into the ‘unknown’ and ‘unfamiliar’ • To safely enjoy unfamiliar terrain, you should “Shoot PARR”: • Be PREPARED – think ahead, have a plan, know your objectives • Be AWARE of your surroundings Locate exits (How do I get out of the room or back to the van?) Locate safety equipment (eye-wash, first-aid kit, extinguisher, etc.) Locate danger areas (boiling water, chemicals, cliffs, rocks)

  15. Travel Safely • Science is an adventure into the ‘unknown’ and ‘unfamiliar’ • To safely enjoy unfamiliar terrain, you should “Shoot PARR”: • Be PREPARED – think ahead, have a plan, know your objectives • Be AWARE of your surroundings Locate exits (How do I get out of the room or back to the van?) Locate safety equipment (eye-wash, first-aid kit, extinguisher, etc.) Locate danger areas (boiling water, chemicals, cliffs, rocks) Locate other people (above me, behind me, next to me)

  16. Travel Safely • Science is an adventure into the ‘unknown’ and ‘unfamiliar’ • To safely enjoy unfamiliar terrain, you should “Shoot PARR”: • Be PREPARED – think ahead, have a plan, know your objectives • Be AWARE of your surroundings Locate exits (How do I get out of the room or back to the van?) Locate safety equipment (eye-wash, first-aid kit, extinguisher, etc.) Locate danger areas (boiling water, chemicals, cliffs, rocks) Locate other people (above me, behind me, next to me) Look where you are going – Don’t BLUNDER AROUND! - Classes can be crowded, with people holding glassware, equipment, fluids, or organisms. Be observant and considerate. - The field has poison ivy, snakes, thorns, and other animals and plants that you want to see, but from a safe distance.

  17. Travel Safely • Science is an adventure into the ‘unknown’ and ‘unfamiliar’ • To safely enjoy unfamiliar terrain, you should “Shoot PARR”: • Be PREPARED – think ahead, have a plan, know your objectives • Be AWARE of your surroundings Locate exits (How do I get out of the room or back to the van?) Locate safety equipment (eye-wash, first-aid kit, extinguisher, etc.) Locate danger areas (boiling water, chemicals, cliffs, rocks) Locate other people (above me, behind me, next to me) Look where you are going – Don’t BLUNDER AROUND! Notice electrical hazards: tabletop outlets and frayed cords

  18. Travel Safely • Science is an adventure into the ‘unknown’ and ‘unfamiliar’ • To safely enjoy unfamiliar terrain, you should “Shoot PARR”: • Be PREPARED – think ahead, have a plan, know your objectives • Be AWARE of your surroundings • Take RESPONSIBILITY – things can be “your fault”, even by inaction

  19. Travel Safely • Science is an adventure into the ‘unknown’ and ‘unfamiliar’ • To safely enjoy unfamiliar terrain, you should “Shoot PARR”: • Be PREPARED – think ahead, have a plan, know your objectives • Be AWARE of your surroundings • Take RESPONSIBILITY – things can be “your fault”, even by inaction Examples: - wear gloves if you think you should, even if you are not told to - Wipe up spills (if you know what it is), use chemical spill kit - sweep broken glass, dispose of correctly - Throw away ‘sharps’ that are lying unattended in ‘sharps’ receptacle - Offer help to classmates and professors - Notify students and professors of potential risks or opportunities - clean up your work station before leaving

  20. Travel Safely • Science is an adventure into the ‘unknown’ and ‘unfamiliar’ • To safely enjoy unfamiliar terrain, you should “Shoot PARR”: • Be PREPARED – think ahead, have a plan, know your objectives • Be AWARE of your surroundings • Take RESPONSIBILITY – things can be “your fault”, even by inaction • Follow the RULES

  21. Basic Rules • NO FOOD OR DRINK IN LAB ROOMS! Yes, this includes your water bottle

  22. Basic Rules • NO FOOD OR DRINK IN LAB ROOMS! • Dress appropriately Closed-toed shoes required Long hair should be pulled back in lab

  23. Basic Rules • NO FOOD OR DRINK IN LAB ROOMS! • Dress appropriately • Reduce Chemical Exposure Skin (especially hands and feet) Wear shoes. Wear lab gloves, lab coats, and aprons when needed If exposed, wash area immediately Wash hands when lab is complete

  24. Sign indicating gloves must be worn. But you may need to wear gloves even if you don’t see this sign. Basic Rules • NO FOOD OR DRINK IN LAB ROOMS! • Dress appropriately • Reduce Chemical Exposure Skin (especially hands and feet) Special Issues with Gloves: - If using solvents, be sure gloves are resistant. - Be aware of nicks & tears; replace as needed. - Most gloves are nitrile; but if you are allergic to latex, check! - As well as protecting your hands, gloves keep you from unconsciously putting your fingers in your mouth or eyes. - If your gloves may be contaminated, don’t leave the lab with them on.

  25. Basic Rules • NO FOOD OR DRINK IN LAB ROOMS! • Dress appropriately • Reduce Chemical Exposure Skin (especially hands and feet) Respiratory (dusts, vapors, gases) work in fume hood

  26. Basic Rules • NO FOOD OR DRINK IN LAB ROOMS! • Dress appropriately • Reduce Chemical Exposure Skin (especially hands and feet) Respiratory (dusts, vapors, gases) Ingestion Never pipette by mouth, keep fingers out of mouth Never drink from lab glassware or containers NO FOOD AND DRINK IN THE LAB ROOMS!

  27. Basic Rules Mandatory eye/face protection • NO FOOD OR DRINK IN LAB ROOMS! • Dress appropriately • Reduce Chemical Exposure Skin (especially hands and feet) Respiratory (dusts, vapors, gases) Ingestion Eyes (very important; permanent injury likely) Wear goggles with caustic chemicals (acids, bases) Keep fingers out of eyes Opt for glasses over contacts for lab – and use goggles!!! USE UVgoggles/shield when using UV light sources

  28. Basic Rules – Eyewash Stations If you can’t see, ask for help to the eyewash station!!! • TYPE 1: In wall • Pull arm down • Water flows For corrosives, wash for 10-15 minutes before seeking medical attention. • TYPE 2: In sink • Pull arm down • Water flows

  29. Basic Rules – Shower The shower handle is by the eyewash station on the wall. Pull handle down Shower if clothes are contaminated and remove clothes

  30. Basic Rules • NO FOOD OR DRINK IN LAB ROOMS! • Dress appropriately • Reduce Chemical Exposure • Do not work alone when performing hazardous tasks

  31. Basic Rules • NO FOOD OR DRINK IN LAB ROOMS! • Dress appropriately • Reduce Chemical Exposure • Do not work alone when performing hazardous tasks • Safety Data Sheets (SDS) are available for all chemicals - Kept in the chemical stockroom, 038. - They provide information on chemical hazards of each chemical - If in doubt, take precautions.

  32. Basic Rules • Hazardous Material Labels

  33. Basic Rules • Hazardous Material Labels:

  34. Basic Rules • Hazardous Material Labels: • Many have CLASSES (1-5): 1 is MOST DANGEROUS • Those in LESS HAZARDOUS classes are labelled with an exclamation sign. SDS: Hydrochloric Acid

  35. Basic Rules • Hazardous Material Labels: • Acute Toxicity: may cause serious health effects or death in small amounts or short exposures

  36. Basic Rules • Hazardous Material Labels: • Corrosive: to metals, skin, and eyes; causes burns and severe irritation

  37. Basic Rules • Hazardous Material Labels: • Fire Hazard: Flammable substances, self-heating mixtures, self-reactive mixtures, or those that produce flammable gases when contacted with water

  38. Basic Rules • Hazardous Material Labels: • Oxidizers: gases, liquids, or solids that can cause combustion

  39. Basic Rules • Hazardous Material Labels: • Cylinder: Contents under pressure

  40. Basic Rules • Hazardous Material Labels: • Explosion Risk: self-reactive substances

  41. Basic Rules • Hazardous Material Labels: • Health Hazard: Respiratory or skin sensitization, Germ cell mutagenicity, Carcinogenicity, Reproductive toxicity, Specific Target Organ Toxicity, Aspiration hazard

  42. Basic Rules • Hazardous Material Labels: • Warning: less hazardous (in THAT category); harmful or an irritant

  43. Basic Rules • Hazardous Material Labels: • Biohazard: infectious materials

  44. Basic Rules • Hazardous Material Labels: • Environmental Hazard: Aquatic toxicity (not a mandatory label)

  45. Basic Rules • Hazardous Material Labels: • Radiation Hazard: These involve special issues and require special training. You are not authorized to work with them until you receive such training.

  46. Basic Rules • NO FOOD OR DRINK IN LAB ROOMS! • Dress appropriately • Reduce Chemical Exposure • Do not work alone when performing hazardous tasks • Safety Data Sheets (SDS) are available for all chemicals • Hazardous Material Labels • DISPOSE OF MATERIALS CORRECTLY!

  47. Basic Rules • Dispose of materials correctly: - Sharps (needles, razor blades) in special bins.

  48. Basic Rules • Dispose of materials correctly: - Sharps (needles, razor blades) in special bins. - Glassin special buckets. Don’t put regular trash in these. Clean Paper Uh… GLASS! ONLY!! Trash (no biological material)

  49. Basic Rules • Dispose of materials correctly: - Sharps (needles, razor blades) in special bins. - Glassin special buckets. Don’t put regular trash in these. - Biological waste (bacteria, infectious materials) must be ‘red-bagged’ (and autoclaved before disposal). Notice ‘Biohazard’ Symbol

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