1 / 10

Biotechnology: Gel Electrophoresis

Explore how Gel Electrophoresis, a biotechnological marvel, revolutionizes DNA fingerprinting to solve mysteries and identify individuals. Learn about its applications in criminal investigations, paternity testing, and bioengineering advancements.

mauricethompson
Download Presentation

Biotechnology: Gel Electrophoresis

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. Biotechnology: Gel Electrophoresis

  2. Using the new to improve the old….. • Our knowledge of the structure and function of DNA is fairly new information. • Watson & Crick - 1953 • However, in a short amount of time, our DNA knowledge has been used to: • Improve identification methods of criminals • Determine maternity/paternity • Bioengineer products • Blood typing was first used for some of these purposes, but it has limited capability because it is not specific to each person.

  3. Gel Electrophoresis (DNA Fingerprinting) • Every person has a unique sequence of DNA • Restriction enzymes can be used to cut a strand of DNA into several pieces • e.g., enzyme cuts when it encounters GAATTC • A DNA fingerprint is a unique “picture” of a person’s cut up DNA fragments that are then separated by size Who took Jimmy's lollipop?

  4. The short version of how DNA fingerprints are made DNA is (-) charged. When you turn on the gel, the DNA samples move across to the (+) side. Because the gel is porous (contains small holes), large fragments get stuck and don’t move far whereas small fragments move farther.

  5. Examples: Interpreting DNA Fingerprints

  6. Example 1: Who left the blood stain? How do you know? http://www.biologycorner.com/resources/DNAfinger.gif

  7. Example 2: Who are the children of these parents http://www.scq.ubc.ca/a-brief-tour-of-dna-fingerprinting/

  8. Example 3: Who is the father? How do you know?

  9. Example 4: Who are the parents of this soldier? How do you know? http://www.glencoe.com/qe/images/b136/q4318/ch13_0_c.gif

More Related