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Isochorismate synthase 欠損株、回復系 微生研　小林　大哲

Isochorismate synthase 欠損株、回復系 微生研　小林　大哲. 挿入変異株 / 回復系作成. Ligation Product. pRK415 / Vector. Wild Type. Ligation. 実験概要 ①. Ｓｅｅｄ、本培養 Qiagen / Genome 抽出 PCR. Enzyme （ SacⅠ,KpNⅠ ）. ＪＭ１０９. Ligation Product. Ｔｒａｎｓｆｏｒｍａｔｉｏｎ. Ｔｒａｎｓｆｏｒｍａｔｉｏｎ. Incubation Qiagen / Plasmid 回収. SM10λ. Ｐｌａｓｍｉｄ.

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Isochorismate synthase 欠損株、回復系 微生研　小林　大哲

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  1. Isochorismate synthase欠損株、回復系 微生研　小林　大哲 挿入変異株/回復系作成

  2. Ligation Product pRK415 / Vector Wild Type Ligation 実験概要① Ｓｅｅｄ、本培養 Qiagen/ Genome抽出 PCR Enzyme（SacⅠ,KpNⅠ）

  3. ＪＭ１０９ Ligation Product Ｔｒａｎｓｆｏｒｍａｔｉｏｎ Ｔｒａｎｓｆｏｒｍａｔｉｏｎ Incubation Qiagen / Plasmid回収 SM10λ Ｐｌａｓｍｉｄ 実験概要② / Plasmid増幅 Filter Mating to Vibrio (Wild Type)

  4. EtOH後、泳動 条件検討 実験結果 • JM109Competent Cellの作製… • LB plate（with X-Gal , ITPG , Tetracyclin）　

  5. 　回復系作成を完了　 • 　操作をきちんと覚える • 　自分ひとりで実験計画し、結果を出せるようになる。 次のData Discussionまで…

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