1 / 5

Absorbance at 280 nm ( mAU )

Fig. S1. A. 180. 100. Absorbance at 280 nm ( mAU ). 20. 15. 10. Elution volume (ml). MW ( kDa ). 6.5 7 13.5 14.5 15 15.5 16 16.5 18.5 19.5. B.

Download Presentation

Absorbance at 280 nm ( mAU )

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript


  1. Fig. S1 A 180 100 Absorbanceat 280 nm (mAU) 20 15 10 Elution volume (ml) MW (kDa) 6.5 7 13.5 14.5 15 15.5 16 16.5 18.5 19.5 B Fig. S1. Gel filtration profile of HsNadE2. A) Elution profile of HsNadE2 on a Superose 6 column, the elution volume corresponds to 85 kDa. B) SDS-PAGE analysis of gel filtration chromatography fractions.

  2. Fig. S2 A B D C F E Fig. S2– Determination of kinetic parameters for NAD synthetases from A. brasilense and H. seropedicae. Initial velocity (Vo) for the substrates ammonium (A,C and E) and glutamine (B, D and F). Average data from triplicate experiments ± SD.

  3. Fig. S3 MW (kDa) 1 2 3 4 5 6 92 67 43 30 Fig. S3- SDS-PAGE analysisofA. brasilense NadE1Gln, NadE2GlnandNadENH3.MW molecular markers. His-tag proteins: AbNadE1Gln-His (Lane 1), AbNadE2Gln-His (Lane 3), AbNadE3NH3-His (Lane 5). Taglessproteins used in this work: AbNadE1Gln (Lane 2), AbNadE2Gln (Lane 4), and AbNadE3NH3 (Lane 6). ). The gel wascoomassiebluestained. Thelineindicatesthesplicing in theimageofthesame gel.

  4. Fig. S4 A 180 100 Absorbanceat 280 nm (mAU) 10 15 20 Elution volume (ml) MW (kDa) 13.5 14 14.5 15 15.5 16 17 19.5 B Fig. S4– Gel filtration profile of AbNadE2. A) Elution profile of AbNadE2 on a Superose 6 column, the elution volume corresponds to 103 kDa. B) SDS-PAGE analysis of gel filtration chromatography fractions.

  5. Fig. S5 Fig. S5– Superposition of the NAD+ synthetase domain of NadE2Gln from Burkholderiathailandensis(red) and NadENH3 from Bacillus subtilis(green). View from the top of the NAD+ synthetase domain. The PDB structures 4FH4 (amino acids 281 to 542) and 1KQP (full length) were aligned and visualized using Pymol.

More Related