1 / 6

Julie Gaertner University of Hawai`i Hilo

Identifying Sources of Microbial Pollution Using Traditional Microbial and Molecular Source Tracking Techniques. Julie Gaertner University of Hawai`i Hilo M.S. Tropical Conservation Biology & Environmental Science. Microbial Methods. Clostridium perfringens. Enterococcus spp.

mick
Download Presentation

Julie Gaertner University of Hawai`i Hilo

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. Identifying Sources of Microbial Pollution Using Traditional Microbial and Molecular Source Tracking Techniques Julie Gaertner University of Hawai`i Hilo M.S. Tropical Conservation Biology & Environmental Science

  2. Microbial Methods Clostridium perfringens Enterococcus spp. Enumerated with Enterolert Kits by IDEXX Laboratories Inc. Photo Credit: Oxiod.com Photo Credit: Santuarysimon.org Enumerated by Membrane Filtration Method Bisson and Cabelli 1979.

  3. Molecular Method Human Specific Genetic Markers Bacteroidales 16S rRNA • Bacteroides-Prevotellagroup • Length heterogeneity PCR • Terminal restriction fragment length polymorphism (T-RFLP) PCRdetection of human-specific Bacteroides genetic marker from sewage(Bower et al. 2005).

  4. Water Quality Parameters Salinity Temperature Specific Conductivity Dissolved Oxygen Turbidity pH Weather & Climate Data • Total Nitrogen • Total Dissolved Nitrogen • Nitrate + Nitrite • Ammonium • Total Phosphorus • Phosphate • Light Levels

  5. High Resolution GIS Mapping CYCLOPS-7 Optical Brightener Fluorometer Probe DataBank Data Logger – GPS Enabled Map Credit: Ambyr-Mokiao-Lee Photo Credit: Turnerdesigns.com

  6. Acknowledgements • Dr. Tracy Wiegner • Dr. Jon Awaya • Dr. Stephanie Molloy • Dr. Misaki Takabayashi • Dr. Mazen Hamad • Ambyr Mokiao-Lee • Eric Johnson • Lucas Mead • Narrissa Spies • Steven Bradshaw • University of Hawai`i Hilo EPSCoR Analytical • Hawai`i Department of Health

More Related