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Half Adder - Modeling Overview

Half Adder - Modeling Overview. September 4th, 2006. ETH Zürich | iGEM team 2006. System Architecture. Light. Light Sensing. PoPS Duplexer. PoPS to GFP. PoPS to RFP. Chemical Sensing. PoPS Duplexer. PoPS. PoPS. PoPS. PoPS. PoPS. PoPS. PoPS. PoPS. Half-Adder. XOR. AND.

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Half Adder - Modeling Overview

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  1. Half Adder - Modeling Overview September 4th, 2006 ETH Zürich | iGEM team 2006

  2. System Architecture Light LightSensing PoPSDuplexer PoPSto GFP PoPSto RFP ChemicalSensing PoPSDuplexer PoPS PoPS PoPS PoPS PoPS PoPS PoPS PoPS Half-Adder XOR AND

  3. Insights Overview

  4. Light Sensing P OmpR OmpR PoPS Light Heme PCB ho1 PcyA PCB PCB Cph1 Cph1 Cph1 EnvZ EnvZ EnvZ P Auto-Phosphorylation Part: BBa_I15010 Cph8: Fusion Cph1/EnvZChimeric Cph1 light receptor/EnvZ protein Part:BBa_I15008 Heme Oxygenase (ho1) Part:BBa_I15009 Phycocyanobilin (PcyA)ferredoxin oxidoreductase Part: BBa_R0082

  5. Chemical Sensing IPTG IPTG IPTG BBa_C0012 LacI LacI LacI repressor BBa_I14032 BBa_R0011 Strong constitutive ON promoter Strong promoter (lacI regulated) BBa_B0031 PoPS RBS Part: BBa_Q01121 BBa_B0012 Terminator BBa_B0010 Terminator

  6. PoPS Duplexer PoPS PoPS PoPS PoPS PoPS PoPS Light PoPS Duplexer D1 D2 Chemical PoPS Duplexer D3 D4

  7. PoPS Duplexer Simplified PoPS PoPS PoPS PoPS PoPS PoPS PoPS PoPS PoPS Duplexing by Concatenation PoPS Duplexer, 1 Inducer a D b a b

  8. AND (tRNA) tRNA PoPS PoPS PoPS AND t7r mT7R T7Rd T7R trna T7R: T7 RNA Polymerase T7Rd: deficient (inactive) T7RmT7R: mRNA for T7R tRNA: tRNA to repress „early stop“ translation

  9. XOR PoPS PoPS PoPS XOR I1 i1 i2’ I1’ I1 - I1‘ I2 - I2‘ I2’ i2 i1’ I2

  10. PoPS to Reporter PoPS PoPS GFP gfp RFP rfp

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